BME103:TEMPwu2

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(Thermal Cycler Engineering)
Current revision (15:24, 16 November 2012) (view source)
(OUR TEAM)
 
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=LAB 2 WRITE-UP=
=LAB 2 WRITE-UP=
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(Please finish by 11/28/2012)
 
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'''Our Proposed Design'''<br>
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'''System Design'''<br>
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<!--- Add results from Week 3 exercise Part 2 --->
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'''Key Features'''<br>
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'''Instructions'''<br>
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<!--- From Week 4 exercise --->
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==Protocols==
==Protocols==
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'''Polymerase Chain Reaction'''<br>
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<!--- Design a new protocol based on your group's new PCR design. Make a step-by-step list of how someone should use your method
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(Add your work from Week 3, Part 1 here)<br>
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Things to consider:
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How should the PCR machine be set up? Does it need to be plugged in?
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How many samples will fit into a single 2-hour run?
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How many replicates should be created per patient?
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What should the final volume of the reaction be?
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Will signal reading be integrated into the PCR machine or remain separate?
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If it is separate, you will need to include instructions on how to use the fluorimeter.
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How should the user calculate the about of signal amplified?
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etc.
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--->
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'''Materials'''
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<!--- Place your two tables "Supplied in the kit" and "Supplied by User" here --->
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'''PCR Protocol'''
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'''DNA Measurement Protocol'''
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'''Flourimeter'''
 
==Research and Development==
==Research and Development==
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<!--- Bonus: explain how Bayesian statistics can be used to assess the reliability of your team's method. Just write the equation using variables that are relevant to your team's new test. You do not need actual numbers --->
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'''Background on Disease Markers'''
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<!--- A description of the diseases and their associated SNP's (include the database reference number and web link) --->
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'''Primer Design'''
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<!--- Include the sequences of your forward and reverse primers. Explain why a disease allele will give a PCR product and the non-disease allele will not. --->
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==Results==
 
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(Your group will add the results of your Fluorimeter measurements here after Week 4)
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'''Illustration'''
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<!--- Include an illustration that shows how your system's primers allow specific amplification of the disease-related SNP --->
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Current revision

BME 103 Fall 2012 Home
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Lab Write-Up 3
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Contents

OUR TEAM

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LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design


Key Features


Instructions





Protocols

Materials


PCR Protocol



DNA Measurement Protocol



Research and Development

Background on Disease Markers



Primer Design



Illustration


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