Name: Student: Elyse Candell
Role(s: Machine Engineer)
LAB 2 WRITE-UP
Thermal Cycler Engineering
Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
The B/W part is a bluetooth/wifi sensor. This is to replace the computer.
The outside of the Thermal Cycler is now made out of Polyurethane Foam Rigid instead of wood.
To make things easier, a smart phone will be able to control the the Thermal Cycler, like the computer did in our experiment. This is a button that will be pressed whenever a person wants to connect the Thermal Cycler either using their network or their bluetooth.
The Polyurethane Foam Rigid is a better material to help keep heat in when the Thermal Cycler is heating up and keep it cool when the Thermal Cycler is cooling down. This helps it to heat up faster and cool down faster, thus promoting faster cycles.
| Supplied in the Kit || Amount
| Test Tubes || 32 ||
| Micropipette (10uL-100uL) || 1 ||
| Disposable Pipette tips || 100 ||
| PCR Reaction Mix || 2 mL ||
| Supplied by User || Amount
|Smartphone or Computer|| 1 ||
| Bluetooth Capability in Phone || 1 ||
| USB Cord with Computer || 1 ||
| Patient Samples || 50 uL per Test Tube ||
DNA Measurement Protocol
Research and Development
Background on Disease Markers
CGC changed to CAC
This SNP is linked to schizophrenia. Schizophrenia is a mental disorder with numerous symptoms, including hallucinations, delusions, and abnormal social behavior. It is usually triggered by extreme anxiety in people who are genetically predisposed.
forward primer: AGGGGGGCCAGGGCCTCAGTG
reverse primer: TGCCTTCCGC A CGAGCCCGTC
The disease allele will give a PCR product because it will attach to the primer in the PCR, thus replicating exponentially. A non-diesease allele will not attach to the primer, so the DNA can not replicate exponentially.