OUR TEAM
 Name: Student: Elyse Candell Role(s: Machine Engineer) |
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LAB 2 WRITE-UP
Thermal Cycler Engineering
Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
System Design
<systemchange.png>
Key Features
Instructions
Protocols
Materials
| Supplied in the Kit | Amount
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| Test Tubes | 32 |
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| Micropipette (10uL-100uL) | 1 |
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| Disposable Pipette tips | 100 |
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| PCR Reaction Mix | 2 mL |
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| Supplied by User | Amount
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| Smartphone or Computer | 1 |
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| Bluetooth Capability in Phone | 1 |
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| USB Cord with Computer | 1 |
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| Patient Samples | 50 uL per Test Tube |
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PCR Protocol
DNA Measurement Protocol
Research and Development
Background on Disease Markers
rs74315509
This SNP is linked to schizophrenia.
http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=74315509
Primer Design
forward primer: AGGGGGGCCAGGGCCTCAGTG
reverse primer: TGCCTTCCGC A CGAGCCCGTC
The disease allele will give a PCR product because it will attach to the primer in the PCR, thus replicating exponentially. A non-diesease allele will not attach to the primer, so the DNA can not replicate exponentially.
Illustration
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BME 103 Fall 2012
