BME103:T930 Group 7

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(OUR TEAM)
(OUR TEAM)
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| [[Image:BME103student.jpg|100px|thumb|Name: Wesley Karlin<br>Role(s)Experimental Protocol Planner]]
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| [[Image:BME103student.jpg|100px|thumb|Name: Wesley Karlin<br>Role(s) Experimental Protocol Planner]]
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| [[Image:BME103student.jpg|100px|thumb|Name: Lauren Edwards<br>Role(s)Experimental Protocol Planner]]
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| [[Image:BME103student.jpg|100px|thumb|Name: Lauren Edwards<br>Role(s) Experimental Protocol Planner]]
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| [[Image:BME103student.jpg|100px|thumb|Name: Raphael Pascua<br>Role(s)Machine Engineers]]
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| [[Image:BME103student.jpg|100px|thumb|Name: Raphael Pascua<br>Role(s) Machine Engineers]]
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| [[Image:BME103student.jpg|100px|thumb|Name: Elyse Candell<br>Role(s)Machine Engineers]]
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| [[Image:BME103student.jpg|100px|thumb|Name: Elyse Candell<br>Role(s) Machine Engineers]]
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| [[Image:BME103student.jpg|100px|thumb|Name: Katey Hemphill<br>Role(s)Research and Design Scientist]]
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| [[Image:BME103student.jpg|100px|thumb|Name: Katey Hemphill<br>Role(s) Research and Design Scientist]]
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Revision as of 14:22, 1 November 2012

BME 103 Fall 2012 Home
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Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
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Contents

OUR TEAM

Name: Wesley KarlinRole(s) Experimental Protocol Planner
Name: Wesley Karlin
Role(s) Experimental Protocol Planner
Name: Lauren EdwardsRole(s) Experimental Protocol Planner
Name: Lauren Edwards
Role(s) Experimental Protocol Planner
Name: Raphael PascuaRole(s) Machine Engineers
Name: Raphael Pascua
Role(s) Machine Engineers
Name: Elyse CandellRole(s) Machine Engineers
Name: Elyse Candell
Role(s) Machine Engineers
Name: Katey HemphillRole(s) Research and Design Scientist
Name: Katey Hemphill
Role(s) Research and Design Scientist

LAB 1 WRITE-UP

(Please finish by 11/7/2012)

Initial Machine Testing

The Original Design
(Add image of the full OpenPCR machine here, from the Week 3 exercise. Write a paragraph description for visitors who have no idea what this is)


Experimenting With the Connections

When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer)

When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)


Test Run

(Write the date you first tested Open PCR and your experience(s) with the machine)




Protocols

Polymerase Chain Reaction

(Add your work from Week 3, Part 1 here)


Flourimeter Measurements

(Add your work from Week 3, Part 2 here)




Research and Development

Specific Cancer Marker Detection - The Underlying Technology

(Add a write-up of the information discussed in Week 3's class)

PCR, or Polymerase Chain Reaction, is a process used to make copies of the same DNA sequences. This process includes a template DNA strand, which serves as the DNA that will be replicated. Primers are also needed to artificially synthesize the DNA strand. Taq polymerase then matches base-pairs, thus replicating the DNA. Magnesium Chloride is also needed because it binds to Taq, allowing it to function. Finally, dNTP’s are the nucleotides, A, t C, and G, that are used to make the new DNA. The process includes the following: 1. Heat to 95 degrees C. This separates the complementary DNA strands 2. Cool to 57 degrees C. This allows the primers to bind to the DNA strand. 3. Heat to 72 degrees C. This allows Taq to extend the DNA copy 4. Repeat A cancer gene will produce a positive result because only when the cancer gene is present will the primer bind to the template DNA. Therefore, the DNA will be replicated exponentially, creating thousands of the same DNA sequence. If there is no cancer gene present, then the primer cannot bind to the template DNA, and the DNA will not be replicated exponentially.


(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)




Results

(Your group will add the results of your Fluorimeter measurements from Week 4 here)


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