OUR TEAM

Name: Nicholas Sterkowitz
Open PCR machine engineer

Name: Dominic Ilardi
Open PCR machine engineer

Name: Alexandra Nazareno
Experimental Protocol Planner

Name: Amanda Sweig
Experimental Protocol Planner

Name: Taylor Deegan
Research and Development(s)

LAB 2 WRITE-UP

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.

System Design

Key Features

Instructions

Materials

Supplied in PCR Kit	Amount
PCR Machine	1
Fluorometer Box	1
Calf Thymus	0.5 mL
SYBR Green (diluted)	1.5 mL
Camera Stand	1
Glass Plates	2
Calibration DNA	0.5 mL
GoTaq Polymerase and Buffer Solution	10 mL
Small Test Tubes	25
Large Test Tubes	25

PCR Protocol

1. Using a micro-pipette, transfer 1.0-1.5 mL of desired DNA sample into at least three test tubes.

2. reagent solution

3. place tubes into holder in PCR machine

4. program cycles on OpenPCR

5. Run reaction

DNA Measurement Protocol

Background on Disease Markers

Primer Design

Illustration