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BME 103 Fall 2012
Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM
Name: Nicholas Sterkowitz Open PCR machine engineer
Name: Dominic Ilardi Open PCR machine engineer
Name: Alexandra Nazareno Experimental Protocol Planner
Name: Amanda Sweig Experimental Protocol Planner
Name: Taylor Deegan Research and Development(s)
LAB 2 WRITE-UP
Thermal Cycler Engineering
Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
System Design
Key Features
Instructions
Protocols
Materials
Supplied in PCR Kit
Amount
PCR Machine
1
Fluorometer Box
1
Calf Thymus
0.5 mL
SYBR Green (diluted)
1.5 mL
Camera Stand
1
Glass Plates
2
Calibration DNA
0.5 mL
GoTaq Polymerase and Buffer Solution
10 mL
Supplied by User
Amount
Smartphone Camera
1
Laptop
1
ImageJ Download
1
Power Supply
1
DNA Sample
At least 3 samples
PCR Protocol
DNA Measurement Protocol
Research and Development
Background on Disease Markers
Primer Design
Illustration