BME103:T930 Group 6 l2

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Revision as of 14:48, 15 November 2012

BME 103 Fall 2012 Home
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Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
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Contents

OUR TEAM

Name: Nicholas SterkowitzOpen PCR machine engineer
Name: Nicholas Sterkowitz
Open PCR machine engineer
Name: Dominic IlardiOpen PCR machine engineer
Name: Dominic Ilardi
Open PCR machine engineer
Name: Alexandra NazarenoExperimental Protocol Planner
Name: Alexandra Nazareno
Experimental Protocol Planner
Name: Amanda SweigExperimental Protocol Planner
Name: Amanda Sweig
Experimental Protocol Planner
Name: Taylor DeeganResearch and Development(s)
Name: Taylor Deegan
Research and Development(s)

LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design


Key Features


Instructions





Protocols

Materials

Supplied in PCR Kit Amount
PCR Machine 1
Fluorometer Box 1
Calf Thymus 0.5 mL
SYBR Green (diluted) 1.5 mL
Camera Stand 1
Glass Plates 2
Calibration DNA 0.5 mL
GoTaq Polymerase and Buffer Solution 10 mL
Small Test Tubes 25
Large Test Tubes 25


Supplied by User Amount
Smartphone Camera 1
Laptop 1
ImageJ Download 1
Power Supply 1
DNA Sample At least 3 samples


PCR Protocol



DNA Measurement Protocol

Research and Development

Background on Disease Markers



Primer Design



Illustration


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