BME103:T930 Group 6 l2

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'''Materials'''
'''Materials'''
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<!--- Place your two tables "Supplied in the kit" and "Supplied by User" here --->
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{| class="wikitable"
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|-
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! Supplied in PCR Kit !! Amount
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|-
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| PCR Machine || 1
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|-
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| Fluorometer Box || 1
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|-
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| Calf Thymus || 0.5 mL
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|-
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| SYBR Green (diluted) || 1.5 mL
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|-
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| Camera Stand || 1
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|-
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| Glass Plates || 2
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| Calibration DNA || 0.5 mL
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|-
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| GoTaq Polymerase and Buffer Solution || 10 mL
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|-
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|}
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'''DNA Measurement Protocol'''
'''DNA Measurement Protocol'''
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==Research and Development==
==Research and Development==

Revision as of 14:33, 15 November 2012

BME 103 Fall 2012 Home
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Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
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Contents

OUR TEAM

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LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design


Key Features


Instructions





Protocols

Materials

Supplied in PCR Kit Amount
PCR Machine 1
Fluorometer Box 1
Calf Thymus 0.5 mL
SYBR Green (diluted) 1.5 mL
Camera Stand 1
Glass Plates 2
Calibration DNA 0.5 mL
GoTaq Polymerase and Buffer Solution 10 mL


PCR Protocol



DNA Measurement Protocol

Research and Development

Background on Disease Markers



Primer Design



Illustration


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