BME103:T930 Group 6

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(Protocols)
(Protocols)
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How Polymerase Chain Reaction (PCR) Works  
How Polymerase Chain Reaction (PCR) Works  
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Polymerase Chain Reaction is a biochemical process the replicates and amplifies a desired sequence of DNA. The original DNA strand is pulled apart and polymerase starts the replication of the complementary strands. This process is continued until there are several million copies of the desired strand so it may be examined.<br>
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Polymerase Chain Reaction is a biochemical process the replicates and amplifies a desired sequence of DNA. The original DNA strand is pulled apart and primer marks the location of the targeted DNA sequence for polymerase to begin replication of the complementary strands. This process is continued until there are an exponential amount of the desired strand, allowing it to be closely examined.The PCR reaction relies on thermal cycling, in which repeated cycles of cooling and heating the samples enable the enzymes to appropriately replicate the targeted strand, and carry out the processes previously described.br>
The Steps:  
The Steps:  

Revision as of 14:36, 1 November 2012

BME 103 Fall 2012 Home
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Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
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Contents

OUR TEAM

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LAB 1 WRITE-UP

(Please finish by 11/7/2012)

Initial Machine Testing

The Original Design
(Add image of the full OpenPCR machine here, from the Week 3 exercise. Write a paragraph description for visitors who have no idea what this is)


Experimenting With the Connections

When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer)

When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)


Test Run

(Write the date you first tested Open PCR and your experience(s) with the machine)




Protocols

Polymerase Chain Reaction

How Polymerase Chain Reaction (PCR) Works

Polymerase Chain Reaction is a biochemical process the replicates and amplifies a desired sequence of DNA. The original DNA strand is pulled apart and primer marks the location of the targeted DNA sequence for polymerase to begin replication of the complementary strands. This process is continued until there are an exponential amount of the desired strand, allowing it to be closely examined.The PCR reaction relies on thermal cycling, in which repeated cycles of cooling and heating the samples enable the enzymes to appropriately replicate the targeted strand, and carry out the processes previously described.br>

The Steps: 1.

Reagent Volume
Temple DNA (20 ng) 0.2 μL
10 μM forward primer 1.0 μL
10 μM reverse primer 1.0 μL
GoTaq master mix 50.0 μL
dH2O 47.8 μL
Total Volume 100.0 μL

Flourimeter Measurements

(Add your work from Week 3, Part 2 here)




Research and Development

Specific Cancer Marker Detection - The Underlying Technology

(Add a write-up of the information discussed in Week 3's class)

(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)




Results

(Your group will add the results of your Fluorimeter measurements from Week 4 here)


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