BME103:T930 Group 5 l2

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Soren Mickelsen Role: Experimental Protocol Planner Name: Lindsey Kirkham Role: Experimental Protocol Planner Name: Connor Culhane Role: Research and Development Specialist Name: Michael D. Smith Role: Open PCR Machine Engineer Name: Ricki Ceton Role: Open PCR Machine Engineer LAB 2 WRITE-UP Thermal Cycler Engineering Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski. System Design Key Features The body frame of the PCR Machine are difficult to remove due to the screws. The screw can easily fall into the machine while trying to remove the sides. Therefore, if the one of the sides did not have screws and slide into place it would make the removal of it much easier. The PCR machine could then be fixed or inspected much easier and there would be no screws that could fall in. Instructions In order to set up the new design of the PCR machine, three of the walls, the body top, and the body bottom would have to be screwed together. The body bottom will be a little longer and the body bottom and two body sides will have a divot in them so the removable wall can slide into place. The body top will be a little shorter in length so that the removable wall can be taken out. Protocols Materials PCR Protocol DNA Measurement Protocol Research and Development Background on Disease Markers A very large disease that is wide spread all around the world is Alzheimer’s. Nearly one in every 85 people around the world has this disease. On average, one’s life will end seven years after the diagnosis. Fewer than three percent of patients live longer than fourteen years after the determination of the presence of Alzheimer’s. Of the type of Alzheimer’s that is autosomal, the DNA produces a protein that is different than the expected one. This misfolded protein causes the disease. The DNA sequence that codes for the specific amino acids that form these mutated proteins are called SNPs. An Alzheimer’s related SNP is called rs429358. It exists on the 19th chromosome of the human genome. More information about this SNP can be found by looking up the reference number, rs429358, on OMIM. The normal sequence appears TGC while the mutated Alzheimer’s sequence will show CGC. The respective amino acid produced changes from Cysteine to Arginine. This change in amino acid, causes the proteins to fold differently. Primer Design The reverse primer for this gene would be: GGCGGCCGCACACGTCCTCCCA. The critical sequence begins at 45,411,950 on the 19th chromosome. Now looking 200 base pairs to the left, a forward primer can be created. In this case it would go as follows: CATCCCAGCCCTTCTCCCGC. Illustration An Illustration of the Amplification of DNA This image displays the location of the DNA in the nucleus and the exponential rate of amplification, 2 raised to the n.