BME103:T930 Group 5

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Soren Mickelsen Role: Experimental Protocol Planner Name: Connor Culhane Role: Research and Development Specialist Name: Michael D. Smith Role: Open PCR Machine Engineer Name: Ricki Ceton Role: Open PCR Machine Engineer Name: Lindsey Kirkham Role: Research and Development Specialist LAB 1 WRITE-UP (Please finish by 11/7/2012) Initial Machine Testing The Original Design Experimenting With the Connections When we unplugged the mounting plate from the Open PCR circuit board, the machine's display shut off and we could no longer see any information. When we unplugged the white wire that connects the Open PCR circuit Board to main heating block the machine was no longer able to be measured accurately. Test Run We first tested open PCR on 25 October 2012. The first experiment went well despite som edifficulty opening the lid and plugging the display in. Our program ran smoothly and we got the data we needed. Protocols Polymerase Chain Reaction (Add your work from Week 3, Part 1 here) Flourimeter Measurements (Add your work from Week 3, Part 2 here) Research and Development Specific Cancer Marker Detection - The Underlying Technology (Add a write-up of the information discussed in Week 3's class) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.) Results (Your group will add the results of your Fluorimeter measurements from Week 4 here)