BME103:T930 Group 4 l2

From OpenWetWare
Jump to navigationJump to search
BME 103 Fall 2012 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help

OUR TEAM

Name: Alex Hoang
Role(s): Open PCR Machine Engineer
Name: Bobby Ryan
Role(s): Open PCR Machine Engineer
Name: Sabrina Freeman
Role(s): Experimental Protocol Planner
Name: Sheanah Conneen
Role(s): Experimental Protocol Planner
Name: Hayden Hilliard
Role(s): Research and Development Scientist

LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design


Key Features


Instructions





Protocols

Materials

Supplied in the Kit Amount
PCR machine 1
Empty sample tubes 30
Pre-mixed reagent 1,000 μL
Gloves (latex) 1 pair
Open PCR software 1 copy
Sample tube carrier 1


Supplied by User Amount
Micro-pipetter 1 (50 μL volume)
Pipette tips As needed
Patient samples 1,000 μL
Waste container 1


PCR Protocol



DNA Measurement Protocol

Research and Development

Background on Disease Markers



Primer Design



Illustration