BME103:T930 Group 4: Difference between revisions
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# Create three copies of each original DNA sample. Be sure to label each container explicitly. | # Create three copies of each original DNA sample. Be sure to label each container explicitly. | ||
# Place samples in the PCR machine. | # Place samples in the PCR machine. | ||
# Start the PCR machine. | |||
** The samples will be heated to 95°C to unwind the helix. | ** The samples will be heated to 95°C to unwind the helix. | ||
** The temperature will drop to 57°C to activate the primer and strand pairing. | ** The temperature will drop to 57°C to activate the primer and strand pairing. | ||
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Eight samples were ran. The first sample was number 87998, from a 48 year old female patient. The copies of this sample were made and ran. The second sample was from number 21822, from a 45 year old male patient. There were three copies made from this sample as well, giving the total eight samples. | |||
'''Flourimeter Protocol'''<br> | '''Flourimeter Protocol'''<br> |
Revision as of 16:11, 12 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design
When we unplugged PCB Board of LCD from PCB of open PCR Circuit Board, the LCD display would turn off. When we unplugged the white wire that connects PCB of open PCR Cicuit Board to the temperature sensor wire, the temperature reading of the machine dropped drastically.
10/25/12 Testing the Open PCR for the first time was more complicated than we expected. Our Open PCR had technical difficulties that hindered its performance. Our samples took over 2 hours to cycle completely compared to other Open PCRs which took about 1 1/2 hours. In the end, the machine performed its task successfully. The software interface of the Open PCR was easy to understand and use. The LCD screen display also made it easy to monitor the progress of the test.
ProtocolsPolymerase Chain Reaction Polymerase Chain Reaction (PCR) is a process used to amplify DNA by making millions of copies of a particular sequence.
The PCR master mix is GoTaq® Colorless Master Mix, a premixed ready-to-use solution containing bacterially derived Taq DNA polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR.
Eight samples were ran. The first sample was number 87998, from a 48 year old female patient. The copies of this sample were made and ran. The second sample was from number 21822, from a 45 year old male patient. There were three copies made from this sample as well, giving the total eight samples. Flourimeter Protocol In order to prep for our measurements we took the following steps:
Research and DevelopmentSpecific Cancer Marker Detection - The Underlying Technology (Add a write-up of the information discussed in Week 3's class) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)
Results
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