BME103:T930 Group 4

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Alex Hoang Role(s): Open PCR Machine Engineer; DNA Measurement Operator Name: Bobby Ryan Role(s): Open PCR Machine Engineer; ImageJ Software Processor Name: Sabrina Freeman Role(s): Experimental Protocol Planner; Sample Preparation & Application Name: Sheanah Conneen Role(s): Experimental Protocol Planner; Data Compiler & Analyzer Name: Hayden Hilliard Role(s): Research and Development Scientist LAB 1 WRITE-UP (Please finish by 11/7/2012) Initial Machine Testing The Original Design https://myasucourses.asu.edu/courses/1/2012Fall-T-BME103-86053-86055-86054/groups/_185910_1//_7694078_1/Open%20PCR.png Experimenting With the Connections When we unplugged PCB Board of LCD from PCB of open PCR Circuit Board, the LCD display would turn off. When we unplugged the white wire that connects PCB of open PCR Cicuit Board to the temperature sensor wire, the temperature reading of the machine dropped drastically. Test Run (Write the date you first tested Open PCR and your experience(s) with the machine) 10/25/12 Testing the Open PCR for the first time was more complicated than we expected. Our Open PCR had technicsl difficulties that hindered its performance. Our samples took over 2 hours to cycle completely compared to other Open PCRs which took about 1 1/2 hours. In the end, the machine performed its task successfully. Protocols Polymerase Chain Reaction (Add your work from Week 3, Part 1 here) Flourimeter Measurements (Add your work from Week 3, Part 2 here) Research and Development Specific Cancer Marker Detection - The Underlying Technology (Add a write-up of the information discussed in Week 3's class) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.) Results (Your group will add the results of your Fluorimeter measurements from Week 4 here)