BME103:T930 Group 3 l2: Difference between revisions
Rohan Kumar (talk | contribs) |
Rohan Kumar (talk | contribs) |
||
Line 117: | Line 117: | ||
8.)This process should be repeated for all samples.<br> | 8.)This process should be repeated for all samples.<br> | ||
9.)Now analyze your pictures with Image J software.<br> | 9.)Now analyze your pictures with Image J software.<br> | ||
10.)Open the software. Using the menu selection we used, analyze> set measurements and chose area integrated density and mean grey value.<br> | 10.)Open the software. Using the menu selection we used, analyze> set measurements and chose area integrated density and mean grey value.<br> 11.)Using the menu select image > color > split channels.<br> | ||
12.) This should have created 3 files: a red, green and blue image. You only need the green image.<br> | |||
13.) Now click the menu bar to activate the oval selection.<br> | |||
14.) Draw an oval around the image of your sample and then select analyze > measure.<br> | |||
15.) Now move the previously drawn oval to an area away from the sample to obtain the noise measurements.<br> | |||
16.) Repeat this process for all of your samples, including the controls. <br> | |||
==Research and Development== | ==Research and Development== |
Revision as of 14:55, 28 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features
Instructions
ProtocolsMaterials
PCR Protocol
DNA Measurement Protocol Research and DevelopmentBackground on Disease Markers 1.) The amyloid Beta precursor protein for Alzheimer's - 2.) Ubiquitin-like Modifier-activating enzyme for Spinal muscular atrophy - Primer Design 1.) Forward primer: CTTC[G]ACCT
Illustration
|