BME103:T930 Group 3 l2

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(Protocols)
(Protocols)
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| Template DNA (20 ng) || 0.2 μL  
| Template DNA (20 ng) || 0.2 μL  
|-
|-
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| 10 μM forward primer ||1.0 μL
+
| Fluorimeter ||1
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|-
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| Open PCR Software || 1
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|-
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| Image J Software || 50
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|-
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| Glass Slides  || 50
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|-
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|} <br>
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{| class="wikitable" border="1 px"
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|-
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! Supplied by User !! Amount
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|-
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| Pippets || 8
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|-
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| Computer || 1
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| 10 μM reverse primer || 1.0 μL
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|-
| 10 μM reverse primer || 1.0 μL
| 10 μM reverse primer || 1.0 μL
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| dH<sub>2</sub>O || 47.8 μL
| dH<sub>2</sub>O || 47.8 μL
|-
|-
-
| <strong>Total Volume</strong> || 100.0 μL
 
|}
|}
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<br>
'''PCR Protocol'''
'''PCR Protocol'''

Revision as of 17:22, 28 November 2012

BME 103 Fall 2012 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
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Image:BME494_Asu_logo.png

Contents

OUR TEAM

Name: Lekha Anantuni Role: R&D
Name: Lekha Anantuni
Role: R&D
Name: Rohan Kumar Role(s)
Name: Rohan Kumar
Role(s)
Name: Kyle Stoneking Role(s)
Name: Kyle Stoneking
Role(s)
Name: Austin Cuaderno Role(s)
Name: Austin Cuaderno
Role(s)
Name: Josh Eger Role(s)
Name: Josh Eger
Role(s)

LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design


Key Features


Instructions





Protocols

Materials

Supplied in the kit Amount
Open PCR Machine 1
Template DNA (20 ng) 0.2 μL
Fluorimeter 1
Open PCR Software 1
Image J Software 50
Glass Slides 50

Supplied by User Amount
Pippets 8
Computer 1
10 μM reverse primer 1.0 μL
10 μM reverse primer 1.0 μL
GoTaq master mix 50.0 μL
dH2O 47.8 μL


PCR Protocol



DNA Measurement Protocol

Research and Development

Background on Disease Markers

1.) The amyloid Beta precursor protein for Alzheimer's -
rs63751263 (http://omim.org/entry/104760#0008)
AGACGGAGGAGATCTCTGAAGTGAAG [A/C] TGGATGCAGAATTCCGACATGACTC

2.) Ubiquitin-like Modifier-activating enzyme for Spinal muscular atrophy -
rs80356547(http://omim.org/entry/314370#0002)
GATGGCGTGGCCAATGCCCTGGACAA [C/T] GTCCATGCCCGTCAGTTTGGAGGCG

Primer Design

1.) Forward primer: CTTC[G]ACCT
Reverse primer: TCCA[G]CTTC

2.) Forward primer: TGTT[A]CAGG
Reverse primer: GGAC[A]TTGT


Illustration

Exponential amplification of a specific gene.


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