OUR TEAM

Name: Lekha Anantuni Role: R&D

Name: Rohan Kumar Role(s)

Name: Kyle Stoneking Role(s)

Name: Austin Cuaderno Role(s)

Name: Josh Eger Role(s)

LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.

System Design

Key Features

Instructions

Protocols

Materials

PCR Protocol

DNA Measurement Protocol

Research and Development

Background on Disease Markers

1.) The amyloid Beta precursor protein for Alzheimer's -
rs63751263 (http://omim.org/entry/104760#0008)
AGACGGAGGAGATCTCTGAAGTGAAG [A/C] TGGATGCAGAATTCCGACATGACTC

2.) Ubiquitin-like Modifier-activating enzyme for Spinal muscular atrophy -
rs80356547(http://omim.org/entry/314370#0002)
GATGGCGTGGCCAATGCCCTGGACAA [C/T] GTCCATGCCCGTCAGTTTGGAGGCG

Primer Design

1.) Forward primer: CTTC[G]ACCT

  Reverse primer: TCCA[G]CTTC 

2.) Forward primer: TGTT[A]CAGG

  Reverse primer: GGAC[A]TTGT 

Illustration