BME103:T930 Group 3 l2: Difference between revisions
(4 intermediate revisions by the same user not shown) | |||
Line 24: | Line 24: | ||
==Thermal Cycler Engineering== | ==Thermal Cycler Engineering== | ||
<br> | |||
Our re-design is based upon the [http://openpcr.org Open PCR] system originally designed by Josh Perfetto and Tito Jankowski.<br> | Our re-design is based upon the [http://openpcr.org Open PCR] system originally designed by Josh Perfetto and Tito Jankowski.<br> | ||
Line 139: | Line 139: | ||
15.) Now move the previously drawn oval to an area away from the sample to obtain the noise measurements.<br> | 15.) Now move the previously drawn oval to an area away from the sample to obtain the noise measurements.<br> | ||
16.) Repeat this process for all of your samples, including the controls. <br> | 16.) Repeat this process for all of your samples, including the controls. <br> | ||
<br> | |||
==Research and Development== | ==Research and Development== | ||
Line 170: | Line 171: | ||
<!--- Include an illustration that shows how your system's primers allow specific amplification of the disease-related SNP ---> | <!--- Include an illustration that shows how your system's primers allow specific amplification of the disease-related SNP ---> | ||
[[Image:DNA Amplification.jpg|200px|Exponential amplification of a specific gene.]]<br> | [[Image:DNA Amplification.jpg|200px|Exponential amplification of a specific gene.]]<br> | ||
<br> | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
|} | |} |
Latest revision as of 13:56, 29 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler Engineering
Key Features The old PCR also required a USB cable in order to send information to the computer and receive the input information on the program. The updated Open PCR is easier to connect to the computer since it does not need to be plugged in directly to the computer but instead connects via bluetooth. These two updates make the Open PCR portable and completely wireless. It is more convenient and easy to use compared to the old design of the PCR. We additionally made our new design of the Open PCR aesthetically pleasing, so it will be available in bright and exciting colors such as red, green and purple. Instructions
Protocols
Materials
PCR Protocol
DNA Measurement Protocol Research and DevelopmentBackground on Disease Markers 1.) The amyloid Beta precursor protein for Alzheimer's - Alzheimer's disease is a form of dementia in which patients experience loss of memory , confusion, as well as other symptoms. The amyloid hypothesis states that Alzheimer's is caused by a buildup of the amyloid protein in the brain. This gene for amyloid Beta precursor protein therefore might reveal to doctors and scientist whether or not an individual will develop Alzheimer's. 2.) Ubiquitin-like Modifier-activating enzyme for Spinal muscular atrophy - Spinal muscular atrophy is a disease caused by a genetic mutation in the SMN1 gene. Individuals that have this disease exhibit various degrees of mobility impairment due to the widespread death of motor neurons. Primer Design 1.) Forward primer: CTTC[G]ACCT
Illustration |