BME103:T930 Group 2 l2: Difference between revisions

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'''Materials'''
'''Materials'''


<!--- Place your two tables "Supplied in the kit" and "Supplied by User" here --->
{| border="1" style="border-collapse:collapse;" style="margin: 1em auto 1em auto;"
|-
! Supplied in the Kit !! Amount
|-
| Open PCR Machine || 1
|-
| DNA Primer  || 16.0 μL
|-
| 10 μM Reverse Primer || 16.0 μL
|-
| GoTaq Master Mix || 800.0 μL
|-
| dH<sub>2</sub>O || 764.8 μL
|-
|Tubes || 16
|-
|Fluorimeter || 1
|-
|Teflon Glass Slides || 16
|}
 




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'''DNA Measurement Protocol'''
'''DNA Measurement Protocol'''


==Research and Development==
==Research and Development==

Revision as of 17:12, 27 November 2012

BME 103 Fall 2012 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help

OUR TEAM

Ryan Sullivan
Research Development Scientist
Miriam Y Acosta
PCR Machine Engineer
Ryan Keeney
PCR Machine Engineer
Juliana Ramos
Experimental Protocol Planner
Aaron Cornejo
Experimental Protocol Planner

LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design


Key Features


Instructions





Protocols

Materials

Supplied in the Kit Amount
Open PCR Machine 1
DNA Primer 16.0 μL
10 μM Reverse Primer 16.0 μL
GoTaq Master Mix 800.0 μL
dH2O 764.8 μL
Tubes 16
Fluorimeter 1
Teflon Glass Slides 16


PCR Protocol



DNA Measurement Protocol

Research and Development

Background on Disease Markers



Primer Design



Illustration