BME103:T930 Group 2 l2
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'''Background on Disease Markers''' | '''Background on Disease Markers''' | ||
| - | + | * Sample A | |
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[[Sickle Cell Anemia]] | [[Sickle Cell Anemia]] | ||
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Chromosome 11- single nucleotide variation | Chromosome 11- single nucleotide variation | ||
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http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=35685286 | http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=35685286 | ||
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| + | * Sample B | ||
[[Sickle Cell Anemia]] | [[Sickle Cell Anemia]] | ||
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Chromosome 11- single nucleotide variation | Chromosome 11- single nucleotide variation | ||
| - | + | http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=34430836 | |
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| - | + | '''Primer Design''' | |
| + | * Sample A | ||
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| + | ''rs35685286 [Homo sapiens]'' | ||
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| + | Primer--CTCCGGGACCTGTCCAACCAT | ||
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| + | Reverse Primer-- GAGGCCCTGGACAGGTTGGTA | ||
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| + | * Sample B | ||
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| + | ''rs34430836 [Homo sapiens]'' | ||
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| + | Primer—ATCACTACCGGACCGAGTGGA | ||
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| + | Reverse Primer—TAGTGATGGCCTGGCTCACCT | ||
Revision as of 22:55, 27 November 2012
 BME 103 Fall 2012
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OUR TEAM
LAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features
Instructions
ProtocolsMaterials
Research and DevelopmentBackground on Disease Markers
rs35685286 [Homo sapiens] GGATGAAGTTGGTGGT--GAGGCCCTGG[A/G]CAGGTTGGTA--TCAAGGTTACAAGAC Chromosome 11- single nucleotide variation http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=35685286
rs34430836 [Homo sapiens] AGGTGCTAGGTGCCTT--TAGTGATGGC[C/G]TGGCTCACCT--GGACAACCTCAAGGG Chromosome 11- single nucleotide variation http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=34430836
rs35685286 [Homo sapiens] Primer--CTCCGGGACCTGTCCAACCAT Reverse Primer-- GAGGCCCTGGACAGGTTGGTA
rs34430836 [Homo sapiens] Primer—ATCACTACCGGACCGAGTGGA Reverse Primer—TAGTGATGGCCTGGCTCACCT
Illustration
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