BME103:T930 Group 2: Difference between revisions
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'''Specific Cancer Marker Detection - The Underlying Technology'''<br> | '''Specific Cancer Marker Detection - The Underlying Technology'''<br> | ||
The r17879961 DNA was sequenced to determine the chain of nucleotides that it is composed of. A section of this DNA shows change in the allele sequence | The r17879961 DNA was sequenced to determine the chain of nucleotides that it is composed of. A section of this DNA shows a change in the allele sequence; one which might indicate cancer. In the sequence, a change from "ATT" to "ACT" is the specific indication marker for cancer. The artificial primer that is introduced to the DNA chain is meant to be a complimentary strand that matches up with the "ACT" and surrounding nucleotide sequence ("TGA"). When the primer attaches successfully, it allows the Taq polymerase to finish attaching nucleotides to the sequence. The number of strands will be doubled in this case, and by using the PCR replication machine, the sample will be heated and cooled in specific intervals. These intervals are meant to manipulate the DNA and do things such as separate a strand, cool it down so it is able to bind, as well as stretch it out. This process will, ultimately, result in exponential DNA replication. If the primer does not attach successfully, then this means the person does not have a cancer-indicating sequence, and it will, therefore, not replicate the DNA exponentially. If the DNA replicates exponentially, then the fluorescent dye will show, and this would indicate a cancerous genome. | ||
The illustration below shows the PCR process when the primer attaches to the DNA and the Taq polymerase and is able to complete the DNA chain. | After the PCR machine replication, the samples were put on a slide and combined with Sybr Green indicator. They were then placed in a dark box and a picture was taken of each sample. The samples pictures were uploaded onto the computer and we were able to use software to measured the amount of green fluorescent dye that was in the replicated DNA. The data in the section below describes the data and the parameters that indicate cancer using this information. | ||
The illustration below shows the PCR process when the primer attaches to the DNA and the Taq polymerase and is able to complete the DNA chain, in coordination with the PCR machine temperature cycles. It is shown that throughout the process, the DNA replicates at an exponential rate. | |||
[[Image:Dna_primerGroup2.png|600px|]] | [[Image:Dna_primerGroup2.png|600px|]] | ||
Revision as of 15:41, 12 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||
OUR AWESOME TEAMLAB 1 WRITE-UPInitial Machine TestingAn Open PCR is a thermo-cycler which utilizes a heating plate and cooling fan to rapidly and precisely change the temperature of a DNA sample. The samples are placed in sample holder close to the heating plate. Using a computer program the PCR goes through a cycle of heat changes for a duration of time in order to facilitate the replication and amplification of the DNA sample.
When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer) When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)
(Write the date you first tested Open PCR and your experience(s) with the machine)
ProtocolsPolymerase Chain Reaction Flourimeter Measurements Research and DevelopmentSpecific Cancer Marker Detection - The Underlying Technology The r17879961 DNA was sequenced to determine the chain of nucleotides that it is composed of. A section of this DNA shows a change in the allele sequence; one which might indicate cancer. In the sequence, a change from "ATT" to "ACT" is the specific indication marker for cancer. The artificial primer that is introduced to the DNA chain is meant to be a complimentary strand that matches up with the "ACT" and surrounding nucleotide sequence ("TGA"). When the primer attaches successfully, it allows the Taq polymerase to finish attaching nucleotides to the sequence. The number of strands will be doubled in this case, and by using the PCR replication machine, the sample will be heated and cooled in specific intervals. These intervals are meant to manipulate the DNA and do things such as separate a strand, cool it down so it is able to bind, as well as stretch it out. This process will, ultimately, result in exponential DNA replication. If the primer does not attach successfully, then this means the person does not have a cancer-indicating sequence, and it will, therefore, not replicate the DNA exponentially. If the DNA replicates exponentially, then the fluorescent dye will show, and this would indicate a cancerous genome.
The illustration below shows the PCR process when the primer attaches to the DNA and the Taq polymerase and is able to complete the DNA chain, in coordination with the PCR machine temperature cycles. It is shown that throughout the process, the DNA replicates at an exponential rate.
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