OUR TEAM

Name: Student Role(s)

Name: Student Role(s)

Name: Student Role(s)

Name: Student Role(s)

Name: Student Role(s)

LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.

System Design

Key Features
Four solar panels will be added to the top of the PCR machine. This will allow the PCR machine to run on its own without having to plug it into an outlet; resulting in it being environmentally friendly while still being able to detect different diseases in DNA samples. A crystalized monitor will also be placed on the PCR's side panel. The monitor will display the current status of the sample that is being used, along with the amount of time left in the process. Eliminating the necessity of a computer during this process.

Instructions
1) Take the pre-cut solar panels and insert them into the modified top of the open PCR machine.
2) Once inserted, let these solar panels charge under the light.
3) To insert the crystalized monitor, screw it into the modified side of the open PCR machine. Before placing the monitor into the side of the PCR machine, make sure to connect the correct wires (directed by color) together in order for the monitor to operate.

Protocols

Materials

PCR Protocol
Before start the experiment be sure that the solar panels on the PCR machine have been charged.

DNA Measurement Protocol

Research and Development

Background on Disease Markers

Primer Design

Illustration