OUR TEAM
 Name: Suhail Hiermandi
Role: Research and Development, Machine Engineer
|
 Name: Andrew Trujillo
Role: Research and Development
|
 Name: Elias Mloukhiyeh
Role: Machine Engineer
|
 Name: Emma Goddery
Role: Experiment Protocol Planner
|
LAB 2 WRITE-UP
Thermal Cycler Engineering
Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
System Design
Key Features
Instructions
Protocols
Materials
| Supplied in the Kit |
Amount
|
| Pipette Kit and Tips |
One Micropipetter, 100 tips
|
| PCR tubes |
50
|
| Primers, nucleotides, polymerase |
One kit, can order refill packages
|
| Instructions |
1 booklet, code to download them online as well.
|
| USB Cord |
1
|
| Power Cord (5 ft) |
1
|
| PCR machine |
1
|
| Adjustable Camera Stand to attach to PCR machine |
1
|
| Supplied by User |
Amount
|
| Screwdriver |
1
|
| Computer |
n/a
|
| DNA |
n/a
|
| Camera/Camera Phone/Webcam |
1
|
PCR Protocol
1.
2.
3.
4.
5.
6.
7
8.
9<.br>10.
DNA Measurement Protocol
1.
2.
3.
4.
5.
6.
7
8.
9<.br>10.
Research and Development
Background on Disease Markers
Primer Design
Illustration
|
BME 103 Fall 2012
