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Revision as of 02:33, 29 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features With the changes incorporated with the re-design of the Open PCR machine, the main design changes were to the LCD display screen, front side wall, and the motherboard. The changes would involve moving the location of the LCD display screen to the front side wall as well as adding buttons to the the wall that allow the Open PCR machine to be programmed instead of inputting the desired parameters into a computer with a USB cable. For the program to be on the machine itself, the motherboard would have to be re-programmed so that a computer connected to the Open PCR machine with a USB would no longer be needed to run the machine. Instructions The instructions on how to build the machine would be relatively the same as the before except for a few small changes. The front side wall would have to have a few more instructions to incorporate the LCD screen on it as well as the buttons used to program the machine. Besides that, the instructions to build the Open PCR machine would be the same as before.
ProtocolsMaterials Cover to go over PCR machine || 1
PCR Protocol
Research and DevelopmentBackground on Disease Markers
http://omim.org/entry/185470#0015
Primer Design
Forward Primer: 5’-GAAGGATGAACCTCAGGAGG-3’ (cancer primer) A diseased allele will give the PCR a product because the primer that is given is specific for the disease. The primer will attach itself to the single stranded DNE at a sequence that matches the primer. If there is a replication as a product of a real time PCR run, then the DNA is positive for the cancer mutation and disease. If no replication takes place then the DNA is negative for the cancer mutation and disease.
Illustration |