BME103:T930 Group 15 l2: Difference between revisions
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| Adjustable Camera Stand to attach to PCR machine || 1 | | Adjustable Camera Stand to attach to PCR machine || 1 | ||
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Cover to go over PCR machine || 1 | |||
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<b>DNA Measurement Protocol</b><br>1.<br>2.<br>3.<br>4.<br>5.<br>6.<br>7<br>8. | <b>DNA Measurement Protocol</b><br>1. Add 100 μl of SYBR green into each PCR tube being tested.<br>2. On the LED screen, activate the fluorescence cycle.<br>3. Set up the camera holder so that it goes under the PCR cover and is set up to view the tray of PCR tubes from the side.<br>4. Add a PCR tube with Calf Thymus DNA and SYBR green for a positive control and a PCR tube with only SYBR green for a negative control.<br>5. Put the PCR cover over the PCR machine. <br>6. Download the fluorescence camera app on a smartphone or computer with a webcam.<br>7. Put the camera or webcam in the camera holder and start the app's software to take pictures.<br>8. Images will directly download to the computer to be analyzed. | ||
==Research and Development== | ==Research and Development== |
Revision as of 11:46, 15 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features
Instructions
ProtocolsMaterials Cover to go over PCR machine || 1
PCR Protocol
Research and DevelopmentBackground on Disease Markers
Primer Design
Illustration
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