BME103:T930 Group 15 l2: Difference between revisions

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<b>DNA Measurement Protocol</br><br>1.<br>2.<br>3.<br>4.<br>5.<br>6.<br>7<br>8.<br>9<.br>10.
<b>DNA Measurement Protocol</b><br>1.<br>2.<br>3.<br>4.<br>5.<br>6.<br>7<br>8.<br>9<br>10.


==Research and Development==
==Research and Development==

Revision as of 11:40, 15 November 2012

BME 103 Fall 2012 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help

OUR TEAM

Name: Suhail Hiermandi
Role: Research and Development, Machine Engineer
Name: Andrew Trujillo
Role: Research and Development
Name: Elias Mloukhiyeh
Role: Machine Engineer
Name: Emma Goddery
Role: Experiment Protocol Planner

LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design


Key Features


Instructions





Protocols

Materials

Supplied in the Kit Amount
Pipette Kit and Tips One Micropipetter, 100 tips
PCR tubes 50
Primers, nucleotides, polymerase One kit, can order refill packages
Instructions 1 booklet, code to download them online as well.
USB Cord 1
Power Cord (5 ft) 1
PCR machine 1
Adjustable Camera Stand to attach to PCR machine 1


Supplied by User Amount
Screwdriver 1
Computer n/a
DNA n/a
Camera/Camera Phone/Webcam 1


PCR Protocol
1. Plug the PCR machine in and wait for the program to load completely.
2. On the LED screen on the top of the PCR machine, follow the steps to set up the cycles needed for the DNA replication.
3. Begin to set up the reactants by:
-Put extracted DNA into a PCR tube. -Add primers to the PCR tube.
-Add nucleotides to the PCR tube.
-Add DNA Taq Polymerase to the PCR tube.
-Place this DNA tube into DNA Thermal Cycler.

4. The cycle set up on the LED screen should be as follows:
-Thermal cycler heats to 95 degrees Celsius for 30 seconds.
-Thermal cycler cools to 57 degrees Celsius for 30 seconds.
-Thermal cycler heats to 72 degrees Celsius for 30 seconds.
-These cycles happen 30 times.





DNA Measurement Protocol
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2.
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7
8.
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10.

Research and Development

Background on Disease Markers



Primer Design



Illustration