BME103:T930 Group 15 l2: Difference between revisions
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<b>PCR Protocol</b><br>1.<br>2.<br>3.<br> | <b>PCR Protocol</b><br>1. Plug the PCR machine in and wait for the program to load completely.<br>2. On the LED screen on the top of the PCR machine, follow the steps to set up the cycles needed for the DNA replication.<br>3. Begin to set up the reactants by: <br><i>-Put extracted DNA into a PCR tube. | ||
-Add primers to the PCR tube.<br> | |||
-Add nucleotides to the PCR tube. <br> | |||
-Add DNA Taq Polymerase to the PCR tube. <br> | |||
-Place this DNA tube into DNA Thermal Cycler. </i><br>4. The cycle set up on the LED screen should be as follows: <br><i>-Thermal cycler heats to 95 degrees Celsius for 30 seconds. <br> | |||
-Thermal cycler cools to 57 degrees Celsius for 30 seconds.<br> | |||
-Thermal cycler heats to 72 degrees Celsius for 30 seconds. <br> | |||
-These cycles happen 30 times. </i> | |||
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Revision as of 11:39, 15 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features
Instructions
ProtocolsMaterials
PCR Protocol
Research and DevelopmentBackground on Disease Markers
Primer Design
Illustration
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