BME103:T930 Group 15: Difference between revisions
Emma Goddery (talk | contribs) |
Emma Goddery (talk | contribs) |
||
Line 72: | Line 72: | ||
'''Flourimeter Measurements'''<br> | '''Flourimeter Measurements'''<br> | ||
Flourimeter Assembly:<br>[[image | Flourimeter Assembly:<br>[[image:BME103_Group15_Flourimeter1.jpg|100px]]<br> | ||
Revision as of 20:19, 14 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design
When we unplugged the LCD display from the circuit board, the machine LED display powered down and did not display anything. When we unplugged the white wire that connects the circuit board to the heat sink, the temperature reading on the LED display screen showed the incorrect temperature. Test Run During the "Test Run" portion of the lab on the 25th of October 2012, the OpenPCR machine did as it was supposed to. It fluctuated the heat of the test samples and it also had the same readings on the computer program as well as the LED display screen of the OpernPCR machine.
ProtocolsPolymerase Chain Reaction The Open PCR machine works by heating a sample of DNA to 95 degrees Celsius to separate the sample into single strands. Then, the machine cools to 57 degrees Celsius so that primers can attach to the strand to serve as a probe to a matching sequence. The primer that was used in our experiment was r17879961, a cancer-associated sequence, that will detect if the DNA sample tested positive for cancer. Then, the machine heats up to 72 degrees Celsius where MgCl12 attaches to Taq enzyme, so that Taq can take the free floating dioxynucleotide triphosphates and attach them to the stand to form two new separate and similar DNA strands. Now if the process produce new strands, then the DNA tested positive. If none were present, the DNA tested negative. The reason behind this is that r17879961 only can bind to a sequence that matches its own on the single stranded DNA. This sequence is 5’-AAACTCTTACACTCCATACAT-3’. The cancer mutation site is located at the 12 base pair, 5’-ACT-3’. If the base pair is a C, it is positive for cancer mutation. The base pair should truly be a T, making the sequence 5’-ATT-3’. The particular cancer that is associated with the r17879961 primer is colon rectal cancer. The information regarding this particular cancer sequence can be found on the National Center for Biotechnology Information, in the file “.0002 LI- Faurmen Syndrome”. A study done in the file tested the C mutation r17879961. The results that were found were that 7.8% of C mutations were found in cancer patients, and that 5.3% of C mutations were in Finland. The results then can show that 7.8% of the cancer patients had a positive test, and of that 7.8%, 5.3% are in Finland.
Flourimeter Measurements
Research and DevelopmentSpecific Cancer Marker Detection - The Underlying Technology (Add a write-up of the information discussed in Week 3's class) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)
Results
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||