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BME 103 Fall 2012
Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM
Name: Jake Lindquist Protocol Planner
Name: Breanna Pratt Protocol Planner
Name: Kirsten Jefferys Open PCR Machine Engineer
Name: Ben Alcorn Open PCR Machine Engineer
Name: Carlos Duarte Research and Design Scientist
Name: Bryce DeSimmone Research and Design Scientist
LAB 2 WRITE-UP
Thermal Cycler Engineering
Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
System Design
Key Features
Instructions
Protocols
Materials
Supplied in the kit
Amount
PCR Assembly
1
Fluorimeter
1
Phone Stand
1
Box
1
Supplied by User
Amount
Samples
3 samples per subject
Positive & Negative controls
1 each
Calibrator (Calif.../water blank)
1 each
Enzyme/Primer mix
Enough for all the samples
Test tubes
1 for each sample, and control
Pippettes
1 for each sample, control, and calibration solution
PCR Protocol
DNA Measurement Protocol
Research and Development
Background on Disease Markers
Primer Design
Illustration