BME103:T930 Group 14

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(Protocols)
(Initial Machine Testing)
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[[Image:Open PCR Solid Works Design.png]]<br>
[[Image:Open PCR Solid Works Design.png]]<br>
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'''Description of Open PCR Device''' <br>
'''Experimenting With the Connections'''<br>
'''Experimenting With the Connections'''<br>
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When we unplugged the LED from the Open PCR circuit board, the machine stop displaying information on the display.
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When we unplugged the LED from the Open PCR circuit board, the machine stop displaying information on the LED screen.
-
When we unplugged the white wire that connects the Open PCR circuit board to Main heat sink, the machine incorrectly displays the temperature as negative 40 degrees Celsius.
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When we unplugged the white wire that connects the Open PCR circuit board to main heat sink, the machine incorrectly displays the temperature as negative 40 degrees Celsius.
'''Test Run'''
'''Test Run'''

Revision as of 12:28, 8 November 2012

BME 103 Fall 2012 Home
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Contents

OUR TEAM

Name: Jake LindquistProtocol Planner
Name: Jake Lindquist
Protocol Planner
Name: Breanna PrattProtocol Planner
Name: Breanna Pratt
Protocol Planner
Name: Kirsten JefferysOpen PCR Machine Engineer
Name: Kirsten Jefferys
Open PCR Machine Engineer
Name: Carlos DuarteResearch and Design Scientist
Name: Carlos Duarte
Research and Design Scientist
Name: Ben AlcronOpen PCR Machine Engineer
Name: Ben Alcron
Open PCR Machine Engineer
Name: Bryce DeSimmoneResearch and Design Scientist
Name: Bryce DeSimmone
Research and Design Scientist

LAB 1 WRITE-UP

(Please finish by 11/14/2012)

Initial Machine Testing

The Original Design


Image:Open PCR Solid Works Design.png


Description of Open PCR Device


Experimenting With the Connections

When we unplugged the LED from the Open PCR circuit board, the machine stop displaying information on the LED screen.

When we unplugged the white wire that connects the Open PCR circuit board to main heat sink, the machine incorrectly displays the temperature as negative 40 degrees Celsius.

Test Run

(Write the date you first tested Open PCR and your experience(s) with the machine)




Protocols

Polymerase Chain Reaction

Reagent Volume
Template DNA (20 ng) .2 μL
10 μM forward primer 1.0 μL
10 μM reverse primer 1.0 μL
GoTaq master mix 50.0 μL
dH2O 47.8 μL
Total Volume 100.0 μL


Fluorimeter Measurements

(Add your work from Week 3, Part 2 here)




Research and Development

Specific Cancer Marker Detection - The Underlying Technology

(Add a write-up of the information discussed in Week 3's class)

(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)




Results

(Your group will add the results of your Fluorimeter measurements from Week 4 here)


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