BME103:T930 Group 13 l2

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Contents

THE A TEAM

Name: Marcus SansoniOpen PCR Machine Engineer
Name: Marcus Sansoni
Open PCR Machine Engineer
Name: Pete MarpleExperimental Protocol Planner
Name: Pete Marple
Experimental Protocol Planner
Name: Michelle LipowiczExperimental Protocol Planner
Name: Michelle Lipowicz
Experimental Protocol Planner
Name: Allen JanisR&D Scientist
Name: Allen Janis
R&D Scientist
Name: Ian BainbridgeR&D Scientist
Name: Ian Bainbridge
R&D Scientist
Name: Tyler BarnesOpen PCR Machine Engineer
Name: Tyler Barnes
Open PCR Machine Engineer

LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
fan.png heat%20sink.png

System Design
The Heat Sink Fan moves air across the metal sheets to cool down the PCR machine and the samples. The Heat Sink removes heat from the PCR machine to cool down the samples. It does this by conducting heat down to the metal sheets that are located beside the heat sink fan.

Key Features
For the Heat Sink Fan, we want to add a second fan to the PCR machine. The reason for adding an addtional fan would be cool the heat sink down more rapidly. We also want to change the heat sink in general by add more surface area to the metal sheets in the center. We would increase the number of sheets vertically. We would add sheets horizontally, reducing space between sheets, to increase the surface area even more.

Instructions
The instructions for assembly would not change. To add the second fan we would have to expand the exterior walls to make room for it. This would make the machine slightly larger, but not change its compact, portable size.





Protocols

Materials


PCR Protocol



DNA Measurement Protocol



Research and Development

Background on Disease Markers



Primer Design



Illustration


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