BME103:T930 Group 13

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Pete Marple Experimental Protocol Planner Name: Michelle Lipowicz Experimental Protocol Planner Name: Allen Janis R&D Scientist Name: Ian Bainbridge R&D Scientist Name: Marcus Sansoni Open PCR Machine Engineer Name: Tyler Barnes Open PCR Machine Engineer LAB 1 WRITE-UP (Please finish by 11/7/2012) Initial Machine Testing The Original Design http://openwetware.org/images/4/41/PCR_Machine.png The orignal machine design from the OpenPCR design team. Used as an afforable tool to amplify a DNA sequence using <A HREF=http://en.wikipedia.org/wiki/Polymerase_chain_reaction> Polymerase Chain Reaction </A> Experimenting With the Connections When we unplugged the LCD display from the circuit board, the machine the LCD display turned off. When we unplugged the white wire that connects the circuit board to the main heating block, the machine the LCD displayed a reading of -40 degrees. Test Run (Write the date you first tested Open PCR and your experience(s) with the machine) Protocols Polymerase Chain Reaction Reagent Volume Template DNA (20 ng) .2 uL 10 uM forward primer 1.0 uL 10 uM reverse primer 1.0 uL GoTaq master mix 50.0 uL dH20 47.8 uL Total Volume 100.0 uL Patient #29341 is female at the age of 53. Patient #23292 is male at the age of 56. Flourimeter Measurements (Add your work from Week 3, Part 2 here) Research and Development Specific Cancer Marker Detection - The Underlying Technology (Add a write-up of the information discussed in Week 3's class) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.) Results (Your group will add the results of your Fluorimeter measurements from Week 4 here)