BME103:T930 Group 12: Difference between revisions
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'''Experimenting With the Connections'''<br> | '''Experimenting With the Connections'''<br> | ||
When we unplugged | When we unplugged the LCD plate from the Open PCR circuit board, the display of the machine turned off (it stopped sending data to the display). The circuit board sends electricity through the wires, therefore if it is not plugged into the circuit board, it will not work. | ||
When we unplugged the white wire that connects | When we unplugged the white wire that connects the Open PCR circuit board to the 16 tube PCR block, the machine would not record the temperature. This defeats the whole purpose of the machine as it needs to heat the tubes to the specific temperatures. | ||
'''Test Run''' | '''Test Run''' | ||
On October 25, 2012, we experimented with the Open PCR. The experience was not pleasant as the machine took an hour and forty minutes to finish the experiment. The time estimate was also incorrect as it fluctuated. Fortunately, the experiment was a success as the machine finished the testing, revealing whether the DNA contained mutations. | |||
(Write the date you first tested Open PCR and your experience(s) with the machine)<br> | (Write the date you first tested Open PCR and your experience(s) with the machine)<br> | ||
Revision as of 12:01, 1 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||
OUR TEAMLAB 1 WRITE-UP(Please finish by 11/7/2012) Initial Machine TestingThe Original Design Experimenting With the Connections When we unplugged the LCD plate from the Open PCR circuit board, the display of the machine turned off (it stopped sending data to the display). The circuit board sends electricity through the wires, therefore if it is not plugged into the circuit board, it will not work. When we unplugged the white wire that connects the Open PCR circuit board to the 16 tube PCR block, the machine would not record the temperature. This defeats the whole purpose of the machine as it needs to heat the tubes to the specific temperatures.
On October 25, 2012, we experimented with the Open PCR. The experience was not pleasant as the machine took an hour and forty minutes to finish the experiment. The time estimate was also incorrect as it fluctuated. Fortunately, the experiment was a success as the machine finished the testing, revealing whether the DNA contained mutations.
(Write the date you first tested Open PCR and your experience(s) with the machine)
ProtocolsPolymerase Chain Reaction (Add your work from Week 3, Part 1 here)
(Add your work from Week 3, Part 2 here)
Research and DevelopmentSpecific Cancer Marker Detection - The Underlying Technology (Add a write-up of the information discussed in Week 3's class) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)
Results(Your group will add the results of your Fluorimeter measurements from Week 4 here)
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