BME103:T930 Group 11 l2: Difference between revisions
Line 150: | Line 150: | ||
<br>Step 1: When replicating is finished, remove the 32 tubes from the PCR Machine, as well as the positive and negative controls. | <br>Step 1: When replicating is finished, remove the 32 tubes from the PCR Machine, as well as the positive and negative controls. | ||
<br>Step 2: Transfer DNA samples to eppendorf tube containing 800 mL of buffer. Use a new tip on the pipette for each transfer of DNA to avoid contamination. Do the same for the all the controls. | <br>Step 2: Transfer DNA samples to eppendorf tube containing 800 mL of buffer. Use a new tip on the pipette for each transfer of DNA to avoid contamination. Do the same for the all the controls. | ||
<br>Step 3: | <br>Step 3: Using the rough side of the slides, place two drops over two holes on the slide of SYBR green. Carefully, using a new tip of a pipette, transfer two drops of a DNA sample on the SYBR green. | ||
<br>Step 4: | <br>Step 4: Place the slide in the black box directly in the ray of the light. | ||
<br>Step 5: Take a photo using the smart phone of the slide. | |||
<br>Step 6: Using a new pipette type that is marked for waste, remove the sample from the slide and dispose of it. | |||
<br>Step 7: Repeat steps 3-6 using a new pipette tip for each new sample or control. | |||
==Research and Development== | ==Research and Development== |
Revision as of 18:56, 28 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringSystem Design Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski. Our new design incorporates some new designs such as software, screen zize, number of testing tube lots, as well as size of heating lid. All of these alterations are made to make the Open PCR system more efficient in terms of its operating system and user-friendly features.
Our most major change to the Open PCR System is the change we made to the read-out screen on the top of the device near the heating lid. This change actually affects a few major components of our system. Not only did we move the screen to the side of the machine, rather than the top, but we also optimized the size of it. This size-change allows users to see the read-outs clearer. We also eliminated the need for a computer (or any outside device, that is) as this new larger screen will also be able to control the machine. Now the user is able to input cycles, temperature, etc. right on the screen instead of needing to plug it into a separate system. This allows for better portability and easier use. We also changed the space of the testing tubes so now more tubes can be tested at once. To do this we lengthened the plate as well as the heating lid entirely across the top of the machine. Removing the screen from this part of the Open PCR System also allowed for this change.
Instructions
ProtocolsMaterials
PCR Protocol
DNA Measurement Protocol
Research and DevelopmentBackground on Disease Markers
Primer Design
Illustration
|