BME103:T930 Group 11 l2

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Student Role(s) Name: Student Role(s) Name: Student Role(s) Name: Student Role(s) Name: Student Role(s) LAB 2 WRITE-UP Thermal Cycler Engineering Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski. System Design Key Features Instructions Protocols Materials Supplied in Kit Open PCR Machine (32) Plastic Test Tubes to Fit into PCR Machine PCR Power Adapter USB Cable Flourimeter (8) Glass Slides Supplied by User Low Retention Adjustable Pipette with Disposable Pipette Tips DNA Solution Positive Control DNA Soltution Negative Control DNA Solution What the DNA Solution Should Consist Of 1 Micro-Liter Forward Primer 1 Micro-Liter Reverse Primer 50 Micro-Liter GoTaq Master Mix .2 Micro-Liter Patient's DNA (Or Controls) 47.8 Micro-Liter Distilled Water PCR Protocol Step 1: Turn on the PCR machine. Step 2: Open up the heated lid on top of the PCR. Step 3: Fill the 32 tubes with 32 patient's DNA. Step 4: DNA Measurement Protocol Research and Development Background on Disease Markers Primer Design Illustration