BME103:T930 Group 11 l2

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Revision as of 23:31, 27 November 2012

BME 103 Fall 2012 Home
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Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
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Contents

OUR TEAM

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LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design


Key Features


Instructions





Protocols

Materials


Supplied in Kit
Open PCR Machine
(32) Plastic Test Tubes to Fit into PCR Machine
PCR Power Adapter
USB Cable
Flourimeter
(8) Glass Slides
Supplied by User
Low Retention Adjustable Pipette with Disposable Pipette Tips
DNA Solution
Positive Control DNA Soltution
Negative Control DNA Solution


What the DNA Solution Should Consist Of
1 Micro-Liter Forward Primer
1 Micro-Liter Reverse Primer
50 Micro-Liter GoTaq Master Mix
.2 Micro-Liter Patient's DNA (Or Controls)
47.8 Micro-Liter Distilled Water

PCR Protocol
Step 1: Turn on the PCR machine.
Step 2: Open up the heated lid on top of the PCR.
Step 3: Fill the 32 tubes with 32 patient's DNA.
Step 4:


DNA Measurement Protocol

Research and Development

Background on Disease Markers



Primer Design



Illustration


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