BME103:T930 Group 11 l2: Difference between revisions
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<br>Step 2: Plug in and turn on the Open PCR machine, connect the USB cable to your computer | <br>Step 2: Plug in and turn on the Open PCR machine, connect the USB cable to your computer | ||
<br>Step 3: On the machine interface, select "DNA replication" and then choose desired number of cycles (at least 30 for good results). | <br>Step 3: On the machine interface, select "DNA replication" and then choose desired number of cycles (at least 30 for good results). | ||
<br>Step 4: Using the Pipette, transfer 32 samples of the patients DNA into each test tube. You should only use 1 Pipette tip for this part of the process. | <br>Step 4: Using the Pipette, transfer 32 samples of the patients DNA into each test tube. You should only use 1 Pipette tip for this part of the process. Also transfer the positive and negative control into separate test tubes. | ||
<br>Step 5: Next, transfer the forward and reverse primers into each of the 32 test tubes. 2 Pipette tips should be used in this part of the process: 1 for all of the forward primer transfers, and 1 for all of the reverse primer transfers. | <br>Step 5: Next, transfer the forward and reverse primers into each of the 32 test tubes. 2 Pipette tips should be used in this part of the process: 1 for all of the forward primer transfers, and 1 for all of the reverse primer transfers. | ||
<br>Step 6: Using a new pipette tip, transfer the GoTaq Master mix into each of the 32 test tubes. | <br>Step 6: Using a new pipette tip, transfer the GoTaq Master mix into each of the 32 test tubes. |
Revision as of 15:46, 28 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features
Instructions
ProtocolsMaterials
PCR Protocol
DNA Measurement Protocol
Research and DevelopmentBackground on Disease Markers
Primer Design
Illustration
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