BME103:T930 Group 11 - Revision history

Kenze Caulfield: /* Protocols */

2012-11-15T16:47:54Z

Protocols

←Older revision		Revision as of 16:47, 15 November 2012
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	[[Image:flourimeter11.jpg]] [[Image:setup11.2.jpg]] Left to Right: Flourimeter in action; Apparatus of flourimeter		[[Image:flourimeter11.jpg]] [[Image:setup11.2.jpg]] Left to Right: Flourimeter in action; Apparatus of flourimeter
-		+	[[Image:WP_000071.jpg]] [[Image:WP_000087_copy.jpg]]

	<br><br>		<br><br>

Benjamin Hook: /* OUR TEAM */

2012-11-15T16:41:01Z

OUR TEAM

←Older revision		Revision as of 16:41, 15 November 2012
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	\| [[Image:kenze copy.jpg\|100px\|thumb\|Name: Kenze Caulfield<br>ImageJ Technician]]		\| [[Image:kenze copy.jpg\|100px\|thumb\|Name: Kenze Caulfield<br>ImageJ Technician]]
	\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Jackie Janssen<br>Protocol]]		\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Jackie Janssen<br>Protocol]]
-	\| [[Image:~~Adam11~~.jpg\|100px\|thumb\|Name: Ben Hook<br>Protocol]]	+	\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Ben Hook<br>Protocol]]
	\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Samantha Boccasini<br>Machine Tester]]		\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Samantha Boccasini<br>Machine Tester]]
	\|}		\|}

Ryelle Avylaine Pattuinan: /* Research and Development */

2012-11-15T07:01:42Z

Research and Development

←Older revision		Revision as of 07:01, 15 November 2012
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	This specific sequence is reported to be susceptible too breast and colorectal cancer. It is also linked to Li-Fraumeni syndrome, Osteosarcoma and even prostate cancer. It was also reported that the non-mutated form of the gene is found in about 98.9% of the population while the mutated form is found in 1.1% of the population. Furthermore, the mutated gene is found in 7.8% of patients with colorectal cancer and 5.3% of the rest of the population without colorectal cancer.		This specific sequence is reported to be susceptible too breast and colorectal cancer. It is also linked to Li-Fraumeni syndrome, Osteosarcoma and even prostate cancer. It was also reported that the non-mutated form of the gene is found in about 98.9% of the population while the mutated form is found in 1.1% of the population. Furthermore, the mutated gene is found in 7.8% of patients with colorectal cancer and 5.3% of the rest of the population without colorectal cancer.

		+	Bayes Rule was also used to determine the relative value of one diagnostic test method over another. The equation for Bayes' Rule is P(A\|B) P(B) = P(A,B).

	http://www2.le.ac.uk/departments/emfpu/genetics/explained/images/PCR-process.gif		http://www2.le.ac.uk/departments/emfpu/genetics/explained/images/PCR-process.gif
	[http://www2.le.ac.uk/departments/emfpu/genetics/explained/images/PCR-process.gif]		[http://www2.le.ac.uk/departments/emfpu/genetics/explained/images/PCR-process.gif]
		+

Ryelle Avylaine Pattuinan: /* Results */

2012-11-15T06:58:17Z

Results

←Older revision		Revision as of 06:58, 15 November 2012
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	\| '''Sample''' \|\| '''Integrated Density''' \|\| '''DNA μg/mL''' \|\| '''Conclusion'''		\| '''Sample''' \|\| '''Integrated Density''' \|\| '''DNA μg/mL''' \|\| '''Conclusion'''
	\|-		\|-
-	\| PCR: Negative Control \|\| 677571 \|\| F6 \|\| G6	+	\| PCR: Negative Control \|\| 677571 \|\| 14.1254862 \|\| No Signal
	\|-		\|-
-	\| PCR: Positive Control \|\| 1695980 \|\| F7 \|\| G7	+	\| PCR: Positive Control \|\| 1695980 \|\| 24.59754216 \|\| Positive
	\|-		\|-
-	\| PCR: Patient 1 ID 12123, rep 1 \|\| 1469271 \|\| F8 \|\| G8	+	\| PCR: Patient 1 ID 12123, rep 1 \|\| 1469271 \|\| 9.5987462 \|\| Negative
	\|-		\|-
-	\| PCR: Patient 1 ID 12123, rep 2 \|\| 983747 \|\| F9 \|\| G9	+	\| PCR: Patient 1 ID 12123, rep 2 \|\| 983747 \|\| 16.548578411 \|\| Negative
	\|-		\|-
-	\| PCR: Patient 1 ID 12123, rep 3 \|\| 748210 \|\| ~~F10~~ \|\| ~~G10~~	+	\| PCR: Patient 1 ID 12123, rep 3 \|\| 748210 \|\| 9.54789621 \|\| Negative
	\|-		\|-
-	\| PCR: Patient 2 ID 21312, rep 1 \|\| 424971 \|\| ~~F11~~ \|\| ~~G11~~	+	\| PCR: Patient 2 ID 21312, rep 1 \|\| 424971 \|\| 11.4587165498 \|\| No Signal
	\|-		\|-
-	\| PCR: Patient 2 ID 21312, rep 2 \|\| 738858 \|\| ~~F12~~ \|\| ~~G12~~	+	\| PCR: Patient 2 ID 21312, rep 2 \|\| 738858 \|\| 10.54881694 \|\| Positive
	\|-		\|-
-	\| PCR: Patient 2 ID 21312, rep 3 \|\| 658764 \|\| ~~F13~~ \|\| ~~G13~~	+	\| PCR: Patient 2 ID 21312, rep 3 \|\| 658764 \|\| 13.54489251896 \|\| No Signal
	\|}		\|}

Ryelle Avylaine Pattuinan: /* Results */

2012-11-15T06:54:39Z

Results

←Older revision		Revision as of 06:54, 15 November 2012
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	\| '''Sample''' \|\| '''Integrated Density''' \|\| '''DNA μg/mL''' \|\| '''Conclusion'''		\| '''Sample''' \|\| '''Integrated Density''' \|\| '''DNA μg/mL''' \|\| '''Conclusion'''
	\|-		\|-
-	\| PCR: Negative Control \|\| E6 \|\| F6 \|\| G6	+	\| PCR: Negative Control \|\| 677571 \|\| F6 \|\| G6
	\|-		\|-
-	\| PCR: Positive Control \|\| E7 \|\| F7 \|\| G7	+	\| PCR: Positive Control \|\| 1695980 \|\| F7 \|\| G7
	\|-		\|-
-	\| PCR: Patient 1 ID 12123, rep 1 \|\| E8 \|\| F8 \|\| G8	+	\| PCR: Patient 1 ID 12123, rep 1 \|\| 1469271 \|\| F8 \|\| G8
	\|-		\|-
-	\| PCR: Patient 1 ID 12123, rep 2 \|\| E9 \|\| F9 \|\| G9	+	\| PCR: Patient 1 ID 12123, rep 2 \|\| 983747 \|\| F9 \|\| G9
	\|-		\|-
-	\| PCR: Patient 1 ID 12123, rep 3 \|\| ~~E10~~ \|\| F10 \|\| G10	+	\| PCR: Patient 1 ID 12123, rep 3 \|\| 748210 \|\| F10 \|\| G10
	\|-		\|-
-	\| PCR: Patient 2 ID 21312, rep 1 \|\| ~~E11~~ \|\| F11 \|\| G11	+	\| PCR: Patient 2 ID 21312, rep 1 \|\| 424971 \|\| F11 \|\| G11
	\|-		\|-
-	\| PCR: Patient 2 ID 21312, rep 2 \|\| ~~E12~~ \|\| F12 \|\| G12	+	\| PCR: Patient 2 ID 21312, rep 2 \|\| 738858 \|\| F12 \|\| G12
	\|-		\|-
-	\| PCR: Patient 2 ID 21312, rep 3 \|\| ~~E13~~ \|\| F13 \|\| G13	+	\| PCR: Patient 2 ID 21312, rep 3 \|\| 658764 \|\| F13 \|\| G13
	\|}		\|}

Tony Nguyen: /* OUR TEAM */

2012-11-15T06:51:33Z

OUR TEAM

←Older revision		Revision as of 06:51, 15 November 2012
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	\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Ryelle Pattuinan<br>Research and Development]]		\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Ryelle Pattuinan<br>Research and Development]]
	\| [[Image:kenze copy.jpg\|100px\|thumb\|Name: Kenze Caulfield<br>ImageJ Technician]]		\| [[Image:kenze copy.jpg\|100px\|thumb\|Name: Kenze Caulfield<br>ImageJ Technician]]
-	\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Jackie Janssen<br>~~Role(s)~~]]	+	\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Jackie Janssen<br>Protocol]]
-	\| [[Image:Adam11.jpg\|100px\|thumb\|Name: Ben Hook<br>~~Role (s)~~]]	+	\| [[Image:Adam11.jpg\|100px\|thumb\|Name: Ben Hook<br>Protocol]]
	\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Samantha Boccasini<br>Machine Tester]]		\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Samantha Boccasini<br>Machine Tester]]
	\|}		\|}

Benjamin Hook: /* OUR TEAM */

2012-11-15T06:32:19Z

OUR TEAM

←Older revision		Revision as of 06:32, 15 November 2012
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	\| [[Image:kenze copy.jpg\|100px\|thumb\|Name: Kenze Caulfield<br>ImageJ Technician]]		\| [[Image:kenze copy.jpg\|100px\|thumb\|Name: Kenze Caulfield<br>ImageJ Technician]]
	\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Jackie Janssen<br>Role(s)]]		\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Jackie Janssen<br>Role(s)]]
-	\| [[Image:~~BME103student~~.jpg\|100px\|thumb\|Name: Ben Hook<br>Role (s)]]	+	\| [[Image:Adam11.jpg\|100px\|thumb\|Name: Ben Hook<br>Role (s)]]
	\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Samantha Boccasini<br>Machine Tester]]		\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Samantha Boccasini<br>Machine Tester]]
	\|}		\|}

Ryelle Avylaine Pattuinan: /* Results */

2012-11-15T06:32:17Z

Results

←Older revision		Revision as of 06:32, 15 November 2012
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	KEY		KEY
-	* '''Sample''' = ~~<!--- explain what "~~sample~~" means --->~~	+	* '''Sample''' = This describes the sample number and the patient.
-	* '''Integrated Density''' = ~~<!--- explain what "~~integrated density~~" means and how you did~~ background ~~subtraction to get this value --->~~	+	* '''Integrated Density''' = This is the integrated density of the drop without that of the background.
-	* '''DNA μg/mL''' = ~~<!--- how you calculated this --->~~	+	* '''DNA μg/mL''' = 2 * IntDen of Sample/IntDen of Calf Thymus (without that of the background)
-	* '''Conclusion''' = ~~<!--- explain~~	+	* '''Conclusion''' = Whether or not the exponential DNA replication occurred.
-		+
-		+
-		+
-		+


	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
	\|}		\|}

Ryelle Avylaine Pattuinan: /* Research and Development */

2012-11-15T06:23:41Z

Research and Development

←Older revision		Revision as of 06:23, 15 November 2012
Line 138:		Line 138:
	This specific sequence is reported to be susceptible too breast and colorectal cancer. It is also linked to Li-Fraumeni syndrome, Osteosarcoma and even prostate cancer. It was also reported that the non-mutated form of the gene is found in about 98.9% of the population while the mutated form is found in 1.1% of the population. Furthermore, the mutated gene is found in 7.8% of patients with colorectal cancer and 5.3% of the rest of the population without colorectal cancer.		This specific sequence is reported to be susceptible too breast and colorectal cancer. It is also linked to Li-Fraumeni syndrome, Osteosarcoma and even prostate cancer. It was also reported that the non-mutated form of the gene is found in about 98.9% of the population while the mutated form is found in 1.1% of the population. Furthermore, the mutated gene is found in 7.8% of patients with colorectal cancer and 5.3% of the rest of the population without colorectal cancer.

-	~~(BONUS points~~: ~~Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc~~. ~~to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA~~. ~~Screen~~-~~captures from the OpenPCR tutorial might be useful~~. ~~Be sure to credit the source if you borrow~~ images.)	+
		+	http://www2.le.ac.uk/departments/emfpu/genetics/explained/images/PCR-process.gif
		+	[http://www2.le.ac.uk/departments/emfpu/genetics/explained/images/PCR-process.gif]
		+

Benjamin Hook: /* Protocols */

2012-11-15T06:19:44Z

Protocols

←Older revision		Revision as of 06:19, 15 November 2012
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	! Total Volume \|\| 100.0 μL		! Total Volume \|\| 100.0 μL
	\|}		\|}
		+
		+	<br><br><b>Charts Two and Three: Vital Patient Statistics</b><br>

←Older revision		Revision as of 16:41, 15 November 2012
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	\| [[Image:kenze copy.jpg\|100px\|thumb\|Name: Kenze Caulfield<br>ImageJ Technician]]		\| [[Image:kenze copy.jpg\|100px\|thumb\|Name: Kenze Caulfield<br>ImageJ Technician]]
	\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Jackie Janssen<br>Protocol]]		\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Jackie Janssen<br>Protocol]]
-	\| [[Image:~~Adam11~~.jpg\|100px\|thumb\|Name: Ben Hook<br>Protocol]]	+	\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Ben Hook<br>Protocol]]
	\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Samantha Boccasini<br>Machine Tester]]		\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Samantha Boccasini<br>Machine Tester]]
	\|}		\|}

←Older revision		Revision as of 06:51, 15 November 2012
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	\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Ryelle Pattuinan<br>Research and Development]]		\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Ryelle Pattuinan<br>Research and Development]]
	\| [[Image:kenze copy.jpg\|100px\|thumb\|Name: Kenze Caulfield<br>ImageJ Technician]]		\| [[Image:kenze copy.jpg\|100px\|thumb\|Name: Kenze Caulfield<br>ImageJ Technician]]
-	\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Jackie Janssen<br>~~Role(s)~~]]	+	\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Jackie Janssen<br>Protocol]]
-	\| [[Image:Adam11.jpg\|100px\|thumb\|Name: Ben Hook<br>~~Role (s)~~]]	+	\| [[Image:Adam11.jpg\|100px\|thumb\|Name: Ben Hook<br>Protocol]]
	\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Samantha Boccasini<br>Machine Tester]]		\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Samantha Boccasini<br>Machine Tester]]
	\|}		\|}

←Older revision		Revision as of 06:32, 15 November 2012
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	\| [[Image:kenze copy.jpg\|100px\|thumb\|Name: Kenze Caulfield<br>ImageJ Technician]]		\| [[Image:kenze copy.jpg\|100px\|thumb\|Name: Kenze Caulfield<br>ImageJ Technician]]
	\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Jackie Janssen<br>Role(s)]]		\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Jackie Janssen<br>Role(s)]]
-	\| [[Image:~~BME103student~~.jpg\|100px\|thumb\|Name: Ben Hook<br>Role (s)]]	+	\| [[Image:Adam11.jpg\|100px\|thumb\|Name: Ben Hook<br>Role (s)]]
	\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Samantha Boccasini<br>Machine Tester]]		\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Samantha Boccasini<br>Machine Tester]]
	\|}		\|}