BME103:T130 Group 9 l2

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Student Role(s) Name: Student Role(s) Name: Student Role(s) Name: Student Role(s) Name: Student Role(s) LAB 2 WRITE-UP Thermal Cycler Engineering Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski. System Design Key Features Instructions Protocols Materials The following materials are provided in the PCR and Fluorimeter kit: Supplied in Kit Amount Open PCR Machine 1 Fluorimeter 1 Phone Stand 1 Box 1 Superhydrophobic Slides 10 CD Software 1 The following materials will need to be supplied by the user: Supplied by User Amount Eppendorf tubes 10 Pipettes 12 Pipette Tips 8 Power source 1 Smartphone with Camera 1 ImageJ Software* 1 PCR Master Mix 800 μL SYBR Green Solution 10 mL DNA Samples 6 DNA Positive Control 1 DNA Negative Control 1 Calf Thymus DNA Sample 1 Water 100 mL Plastic Tube-holding Grid 1 Note that the PCR machine does not require a computer but ImageJ does. A further improvement to this technology would be to design an app for ImageJ that can be used on smartphones. PCR Protocol In order to perform PCR, the samples must first be prepared. This is done by adhering to the following steps: 1. Open up all Eppendorf tubes that will be needed to perform the PCR. Place them in the plastic grid to hold them. 2. Fill each Eppendorf tube with 50 μL of the PCR Master Mix using a pipette. 3. Using a distinct, clean pipette tip per sample (including positive and negative controls), fill each tube with one DNA sample. Be sure to close each tube as you fill it with the DNA sample and label the lid. Throw away each pipette tip and place a new one on each time you are going to use a different sample to avoid contamination. Now that the samples haT DNA Measurement Protocol Research and Development Background on Disease Markers Primer Design Illustration