BME103:T130 Group 9 l2

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Student Role(s) Name: Student Role(s) Name: Student Role(s) Name: Student Role(s) Name: Student Role(s) LAB 2 WRITE-UP Thermal Cycler Engineering Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski. System Design Key Features Instructions Protocols Materials The following materials are provided in the PCR and Fluorimeter kit: Supplied in Kit Amount Open PCR Machine 1 Fluorimeter 1 Phone Stand 1 Box 1 Superhydrophobic Slides 10 CD Software 1 The following materials will need to be supplied by the user: Supplied by User Amount Eppendorf tubes 10 Pipettes 12 Pipette Tips 8 Power source 1 Smartphone with Camera 1 ImageJ Software* 1 PCR Master Mix 800 μL Buffer Mix 10 mL DNA Samples 6 DNA Positive Control 1 DNA Negative Control 1 Calf Thymus DNA Sample 1 Water 100 mL Note that the PCR machine does not require a computer but ImageJ does. A further improvement to this technology would be to design an app for ImageJ that can be used on smartphones. PCR Protocol DNA Measurement Protocol Research and Development Background on Disease Markers Primer Design Illustration