BME103:T130 Group 7 l2

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Emily Thompson Research & Development Scientist Name: Vivian Benjes Experimental Protocol Planner/ Data Analyst Name: Frances Marrett Experimental Protocol Planner Name: Chris Glass Open PCR Machine Engineer Name: Ryan Frantz Open PCR Machine Engineer [1] Name: Cenric Nigbur Ghost/ TBD LAB 2 WRITE-UP Thermal Cycler Engineering Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski. System Design Correctly positioning the heating plate on the plastic sample containers proved to be difficult. To make this product more user friendly a button would be added to the heating plate. When in contact with the samples the button would signal the user via the LED display that the heating lid was correctly positioned. This picture of the roof assembly shows where the button will be added. The blue circle would contain the button that would, when in contact with the plastic containers below, signal to the user that the heating plate was correctly positioned. This is another image of where the button would be positioned. The relative size is also accurate as it would need to be able to detect a sample anywhere in the heating block. The original PCR machine was make of lightweight balsa wood. While this was inexpensive it was prone to splitting along the grain and staining. The new PCR machine would be made with a stronger plastic body that would better resist wear and tear and resist becoming stained with liquids commonly seen in a lab. The original PCR machine had a cord that was slightly short and required an extension cord in some cases. The new PCR machine would have a longer cord so as to avoid this problem. The original PCR machine had a limited storage capacity that proved to be a problem in our large class. To make the product more attractive to educators the new machine would have double the capacity. To help minimize the cost of this addition the new product would use the same PCR blocks as before, merely double the number. To accommodate the extra load the heater and cooler would likewise need to be doubled. The original PCR machine was slightly difficult to understand, again to make the product more attractive to educators the new product would have key parts engraved with their names. This would allow a student to quickly identify pieces and deduce their function. No picture is shown for this proposed addition. Key Features 1.Button to display when the heating lid is correctly positioned 2.New plastic housing to replace wood 3.Longer Power Cord 4.Doubled storage capacity 5.Key parts engraved with names (for educational purposes) Instructions Heating Plate Button: This device will need to be attached to the heating plate with provided metal hardware to resist the heat generated. The button will have leads that connect to the main CPU and allow the button to display when the heating lid is in place Plastic Housing: The new housing will be installed in the same manner that the old housing was. Longer Power Cord: The longer cord will be installed in the same manner that the old housing was. Doubled PCR Blocks: This addition will require two PCR blocks to be installed instead of only one. Both will connect to the same CPU and carry out the same function at the same time however. The new PCR block will require the addition of a new heater and cooling unit that will be installed in the same manner that the old units were installed. Engraved Parts: These parts will be installed in the same manner that the old parts were. Protocols Materials Supplied in the Kit Thermal Cycler for PCR reaction Qiagen Fast Cycling PCR kit (50) BioRad Real Time PCR Applications Guide Supplied by User Sterile gloves Access to 120V electrical outlet 10 uL pipette Eppendorf tube block PCR Protocol DNA Measurement Protocol Research and Development Background on Disease Markers Alzheimer's disease, a form of dimentia in which brain function is lost gradually over time, is associated with the SNP rs121918396. The sequence associated with Alzheimer's is TAG, while a normal sequence is TGG. Primer Design Illustration