BME103:T130 Group 7 l2

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Emily Thompson Research & Development Scientist Name: Vivian Benjes Experimental Protocol Planner/ Data Analyst Name: Frances Marrett Experimental Protocol Planner Name: Chris Glass Open PCR Machine Engineer Name: Ryan Frantz Open PCR Machine Engineer [1] Name: Cenric Nigbur Ghost/ TBD LAB 2 WRITE-UP Thermal Cycler Engineering Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski. System Design Correctly positioning the heating plate on the plastic sample containers proved to be difficult. To make this product more user friendly a button would be added to the heating plate. When in contact with the samples the button would signal the user via the LED display that the heating lid was correctly positioned. This picture of the roof assembly shows where the button will be added. The blue circle would contain the button that would, when in contact with the plastic containers below, signal to the user that the heating plate was correctly positioned. This is another image of where the button would be positioned. The relative size is also accurate as it would need to be able to detect a sample anywhere in the heating block. Key Features Instructions Protocols Materials Supplied in the Kit Thermal Cycler for PCR reaction Qiagen Fast Cycling PCR kit (50) BioRad Real Time PCR Applications Guide Supplied by User Sterile gloves Access to 120V electrical outlet 10 uL pipette Eppendorf tube block PCR Protocol DNA Measurement Protocol Research and Development Background on Disease Markers Alzheimer's disease, a form of dimentia in which brain function is lost gradually over time, is associated with the SNP rs121918396. The sequence associated with Alzheimer's is TAG, while a normal sequence is TGG. Primer Design Illustration