BME103:T130 Group 7 l2

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Revision as of 16:50, 27 November 2012

BME 103 Fall 2012 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
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Image:BME494_Asu_logo.png

Contents

OUR TEAM

Name: Emily ThompsonResearch & Development Scientist
Name: Emily Thompson
Research & Development Scientist
Name: Vivian BenjesExperimental Protocol Planner/ Data Analyst
Name: Vivian Benjes
Experimental Protocol Planner/ Data Analyst
Name: Frances MarrettExperimental Protocol Planner
Name: Frances Marrett
Experimental Protocol Planner
Name: Chris GlassOpen PCR Machine Engineer
Name: Chris Glass
Open PCR Machine Engineer
Name: Ryan FrantzOpen PCR Machine Engineer[1]
Name: Ryan Frantz
Open PCR Machine Engineer
[1]
Name: Cenric NigburGhost/ TBD
Name: Cenric Nigbur
Ghost/ TBD

LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design

Image:Roof Assembly small.png Image:Plate with Button.png Image:Plastic Body Final.png Image:Power Cord Final.png Image:32 wells final.png

Key Features


Instructions





Protocols

Materials


Supplied in the Kit

Thermal Cycler for PCR reaction
Qiagen Fast Cycling PCR kit (50)
BioRad Real Time PCR Applications Guide


Supplied by User

Sterile gloves
Access to 120V electrical outlet
10 uL pipette
Eppendorf tube block

PCR Protocol



DNA Measurement Protocol

Research and Development

Background on Disease Markers
Alzheimer's disease, a form of dimentia in which brain function is lost gradually over time, is associated with the SNP rs121918396. The sequence associated with Alzheimer's is TAG, while a normal sequence is TGG.



Primer Design



Illustration


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