BME103:T130 Group 7 l2: Difference between revisions

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<!--- Place your two tables "Supplied in the kit" and "Supplied by User" here --->
<!--- Place your two tables "Supplied in the kit" and "Supplied by User" here --->
'''Supplied in the Kit'''<br>
'''Supplied in the Kit'''<br>
Open PCR - redesigned machine <br>
[http://www.qiagen.com/products/pcr/fastcycling/qiagenfastcyclingpcrkit.aspx Qiagen Fast Cycling PCR kit (50)] <br>
[http://www.qiagen.com/products/pcr/fastcycling/qiagenfastcyclingpcrkit.aspx Qiagen Fast Cycling PCR kit (50)] <br>
[http://www.bio-rad.com/prd/en/US/adirect/biorad?cmd=catProductDetail&vertical=LSE&country=US&lang=en&productID=170-9799EDU BioRad Real Time PCR Applications Guide]<br>
[http://www.bio-rad.com/prd/en/US/adirect/biorad?cmd=catProductDetail&vertical=LSE&country=US&lang=en&productID=170-9799EDU BioRad Real Time PCR Applications Guide]<br>
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'''Supplied by User'''<br>
'''Supplied by User'''<br>
Sterile gloves <br>
Sterile gloves <br>
Access to 120V electrical outlet<br>
Access to 120V electrical outlet<br>
[http://www.sigmaaldrich.com/catalog/product/sigma/z717290?lang=en&region=US 10 uL pipette]<br>
[http://www.sigmaaldrich.com/catalog/product/sigma/z717290?lang=en&region=US 10 uL pipette]<br>
Eppendorf tube block <br>


'''PCR Protocol'''
'''PCR Protocol'''

Revision as of 15:33, 15 November 2012

BME 103 Fall 2012 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help

OUR TEAM

Name: Emily Thompson
Research & Development Scientist
Name: Vivian Benjes
Experimental Protocol Planner/ Data Analyst
Name: Frances Marrett
Experimental Protocol Planner
Name: Chris Glass
Open PCR Machine Engineer
Name: Ryan Frantz
Open PCR Machine Engineer
[1]
Name: Cenric Nigbur
Ghost/ TBD

LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design


Key Features


Instructions





Protocols

Materials


Supplied in the Kit

Open PCR - redesigned machine
Qiagen Fast Cycling PCR kit (50)
BioRad Real Time PCR Applications Guide


Supplied by User

Sterile gloves
Access to 120V electrical outlet
10 uL pipette
Eppendorf tube block

PCR Protocol



DNA Measurement Protocol

Research and Development

Background on Disease Markers



Primer Design



Illustration