BME103:T130 Group 7
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design
When we unplugged the PCB board from the Open PCR circuit board the machine's LED display ceased to function. When we unplugged the white wire that connects the Open PCR circuit board to 16 tube PCR block, the machine's LED display read -40 Celsius indicating that the temperature could not be read.
(Write the date you first tested Open PCR and your experience(s) with the machine)
ProtocolsPolymerase Chain Reaction (Add your work from Week 3, Part 1 here)
(Add your work from Week 3, Part 2 here)
Research and DevelopmentSpecific Cancer Marker Detection - The Underlying Technology Polymerase Chain Reaction, or PCR, is used to ampllify a specific segment of DNA, in this case the segment known to code for cancer. A primer, which is a piece of complementary DNA artificially synthesized from free bases, binds to the desired DNA, and taq polymerase catalyzes the replication of the DNA strand. This is repeated over and over to produce many copies of the DNA. Once the copies are made, a fluorescent dye that only bonds to DNA double strands is added to the solution. If a solution that shows fluorescence by using a fluorimeter, then that DNA sample contains the cancer-associated sequence. The single nucleotide polymorphism, or SNP, that is linked to cancer is rs17879961. The sequence associated with cancer is ACT, while the non-cancer sequence is ATT. PCR works to identify ACT from ATT because the primers, which are complementary to DNA strands containing cancer-positive ACT, will not bind to DNA containing ATT, and instead of DNA double strands, the solution will only contain single strands. The fluorescent dye will only bind to double strands and will identify those.
Results(Your group will add the results of your Fluorimeter measurements from Week 4 here)
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