BME103:T130 Group 6 l2 - Revision history

Samuel T. Zimmerman: /* Thermal Cycler Engineering */

Samuel T. Zimmerman — Fri, 30 Nov 2012 01:52:13 GMT

Thermal Cycler Engineering

←Older revision		Revision as of 01:52, 30 November 2012
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	'''Key Features'''<br>		'''Key Features'''<br>
-	The key feature we modified was the main heating block. We increased the number of samples it can hold from 16 to 64. Because of the increased size of the heating block, the overall size of the PCR Machine is increased. As you can see in the picture, the redesigned PCR machine is quite a bit larger than the original but its footprint is still small enough to be easy to move and easily fit on a countertop.	+	The key feature we modified was the main heating block. We increased the number of samples it can hold from 16 to 64. Because of the increased size of the heating block, the overall size of the PCR Machine is increased. As you can see in the picture, the redesigned PCR machine is quite a bit larger than the original but its footprint is still small enough to be easy to move and easily fit on a countertop. The heating lid is also equipped with raised tabs such that when the heating lid is lowered on to the samples it stops before crushing the tubes.

Samuel T. Zimmerman: /* Thermal Cycler Engineering */

Samuel T. Zimmerman — Fri, 30 Nov 2012 01:48:11 GMT

Thermal Cycler Engineering

←Older revision		Revision as of 01:48, 30 November 2012
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	[[Image:OpenPCR Before After.png\|800x400px]]<br>		[[Image:OpenPCR Before After.png\|800x400px]]<br>
		+	To the left is the original design of the OpenPCR machine and it only has 16 slots for test tubes which amounts to four patients. On the right is our redesign which has 64 slots which amounts to 16 patients.

	'''Key Features'''<br>		'''Key Features'''<br>
-	The key feature we modified was the main heating block. We increased the number of samples it can hold from 16 to 64. Because of the increased size of the heating block, the overall size of the PCR Machine is increased. As you can see in the picture, the redesigned PCR machine is quite a bit larger than the original but still small enough to be easy to move and fit on a countertop.	+	The key feature we modified was the main heating block. We increased the number of samples it can hold from 16 to 64. Because of the increased size of the heating block, the overall size of the PCR Machine is increased. As you can see in the picture, the redesigned PCR machine is quite a bit larger than the original but its footprint is still small enough to be easy to move and easily fit on a countertop.

Adam Helland: /* Thermal Cycler Engineering */

Adam Helland — Thu, 29 Nov 2012 20:46:25 GMT

Thermal Cycler Engineering

←Older revision		Revision as of 20:46, 29 November 2012
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	'''Key Features'''<br>		'''Key Features'''<br>
-	The key feature we modified was the main heating block. We increased the number of samples it can hold from 16 to 64. Because of the increased size of the heating block ~~that also has an effect on~~ the overall size of the PCR Machine. As you can see in the picture, the redesigned PCR machine is quite a bit larger than the original but still small enough to be easy to move and fit on a countertop.	+	The key feature we modified was the main heating block. We increased the number of samples it can hold from 16 to 64. Because of the increased size of the heating block, the overall size of the PCR Machine is increased. As you can see in the picture, the redesigned PCR machine is quite a bit larger than the original but still small enough to be easy to move and fit on a countertop.

Adam Helland: /* OUR TEAM */

Adam Helland — Thu, 29 Nov 2012 20:41:49 GMT

OUR TEAM

←Older revision		Revision as of 20:41, 29 November 2012
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	\| [[Image:BME103_Group6_‎Ryan.png\|100px\|thumb\|Name: Ryan Uchimura<br>Role: Experimental Protocol Planner/Coolest person ever]]		\| [[Image:BME103_Group6_‎Ryan.png\|100px\|thumb\|Name: Ryan Uchimura<br>Role: Experimental Protocol Planner/Coolest person ever]]
	\| [[Image:Sam z pic.png\|100px\|thumb\|Name: Sam Zimmerman<br>Role: OpenPCR Machine Engineer)]]		\| [[Image:Sam z pic.png\|100px\|thumb\|Name: Sam Zimmerman<br>Role: OpenPCR Machine Engineer)]]
-	\| [[Image:~~BME103student~~.~~jpg~~\|100px\|thumb\|Name: Adam Helland<br>Role:]]	+	\| [[Image:AdamHelland.jpg‎\|100px\|thumb\|Name: Adam Helland<br>Role:]]
	\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Dakota Styck<br>Role:]]		\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Dakota Styck<br>Role:]]
	\|}		\|}

Ryan Uchimura: /* Protocols */

Ryan Uchimura — Thu, 29 Nov 2012 20:40:05 GMT

Protocols

←Older revision		Revision as of 20:40, 29 November 2012
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	</tr>		</tr>
	<tr>		<tr>
-	<td>Pipets - 8</td>	+	<td>Pipets - 16</td>
	<td>Smartphone holder - 1</td>		<td>Smartphone holder - 1</td>
	</tr>		</tr>
	<tr>		<tr>
-	<td>PCR reaction mix - 8 tubes, 50 μL each</td>	+	<td>PCR reaction mix - 16 tubes, 50 μL each</td>
	<td></td>		<td></td>

Line 102:		Line 102:
	</tr>		</tr>
	<tr>		<tr>
-	<td>DNA - 8 samples</td>	+	<td>DNA - 16 samples</td>
	<td>Image J Software - 1</td>		<td>Image J Software - 1</td>
	</tr>		</tr>

Adam Helland: /* Research and Development */

Adam Helland — Thu, 29 Nov 2012 20:39:59 GMT

Research and Development

←Older revision		Revision as of 20:39, 29 November 2012
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	<!--- Include an illustration that shows how your system's primers allow specific amplification of the disease-related SNP --->		<!--- Include an illustration that shows how your system's primers allow specific amplification of the disease-related SNP --->
-	[[Image:Pcrdiagram.gif‎\|~~800x400px~~]]<br>	+	[[Image:Pcrdiagram.gif‎\|464×598px]]<br>

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Adam Helland: /* Research and Development */

Adam Helland — Thu, 29 Nov 2012 20:39:01 GMT

Research and Development

←Older revision		Revision as of 20:39, 29 November 2012
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	<!--- Include an illustration that shows how your system's primers allow specific amplification of the disease-related SNP --->		<!--- Include an illustration that shows how your system's primers allow specific amplification of the disease-related SNP --->
-		+	[[Image:Pcrdiagram.gif‎\|800x400px]]<br>

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Ryan Uchimura: /* Protocols */

Ryan Uchimura — Thu, 29 Nov 2012 20:35:23 GMT

Protocols

←Older revision		Revision as of 20:35, 29 November 2012
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	'''PCR Protocol'''		'''PCR Protocol'''
-		+	<br>1. Connect PCR to computer and install software.<br>
		+	2. Create a new program <br>
		+	set initial step to 95°C for 30 seconds, set for 30 cycles<br>
		+	denaturing- set temperature to 95°C for 30 seconds<br>
		+	annealing- set temperature to 57°C for 30 seconds<br>
		+	extension- set temperature to 72°C for 180 seconds, set to hold at 4°C<br>
		+	3. collect DNA samples
		+	4. mix each DNA sample with individual PCR solution (one pipet per sample)
		+	5. place DNA mixed with the PCR solution in the PCR machine
		+	6. once PCR is complete extract samples


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	<font face="century gothic">		<font face="century gothic">
		+
	==Research and Development==		==Research and Development==

Samuel T. Zimmerman: /* Thermal Cycler Engineering */

Samuel T. Zimmerman — Thu, 29 Nov 2012 20:28:38 GMT

Thermal Cycler Engineering

←Older revision		Revision as of 20:28, 29 November 2012
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	'''Instructions'''<br>		'''Instructions'''<br>
-		+	As our redesign only modified the size of the OpenPCR machine, the assembly instructions are identical to the original assembly instructions which can be found on the [http://openpcr.org/build-it/ OpenPCR Website]

	<!--- From Week 4 exercise --->		<!--- From Week 4 exercise --->

Samuel T. Zimmerman: /* Thermal Cycler Engineering */

Samuel T. Zimmerman — Thu, 29 Nov 2012 19:25:37 GMT

Thermal Cycler Engineering

←Older revision		Revision as of 19:25, 29 November 2012
Line 37:		Line 37:

	'''Key Features'''<br>		'''Key Features'''<br>
-		+	The key feature we modified was the main heating block. We increased the number of samples it can hold from 16 to 64. Because of the increased size of the heating block that also has an effect on the overall size of the PCR Machine. As you can see in the picture, the redesigned PCR machine is quite a bit larger than the original but still small enough to be easy to move and fit on a countertop.

←Older revision		Revision as of 20:41, 29 November 2012
Line 16:		Line 16:
	\| [[Image:BME103_Group6_‎Ryan.png\|100px\|thumb\|Name: Ryan Uchimura<br>Role: Experimental Protocol Planner/Coolest person ever]]		\| [[Image:BME103_Group6_‎Ryan.png\|100px\|thumb\|Name: Ryan Uchimura<br>Role: Experimental Protocol Planner/Coolest person ever]]
	\| [[Image:Sam z pic.png\|100px\|thumb\|Name: Sam Zimmerman<br>Role: OpenPCR Machine Engineer)]]		\| [[Image:Sam z pic.png\|100px\|thumb\|Name: Sam Zimmerman<br>Role: OpenPCR Machine Engineer)]]
-	\| [[Image:~~BME103student~~.~~jpg~~\|100px\|thumb\|Name: Adam Helland<br>Role:]]	+	\| [[Image:AdamHelland.jpg‎\|100px\|thumb\|Name: Adam Helland<br>Role:]]
	\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Dakota Styck<br>Role:]]		\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Dakota Styck<br>Role:]]
	\|}		\|}