BME103:T130 Group 6: Difference between revisions
Line 61: | Line 61: | ||
<li>4. Extension- The DNA replicates to produce millions of copies of the one strand of DNA .</li></ul> | <li>4. Extension- The DNA replicates to produce millions of copies of the one strand of DNA .</li></ul> | ||
<br><br><b>3.</b> The GoTaq master mix contains 400µM dATP, 400µM dGTP, 400µM dCTP, 400µM dTTP, and 3mM MgCl<sub>2</sub> | <br><br><b>3.</b> The GoTaq master mix contains 400µM dATP, 400µM dGTP, 400µM dCTP, 400µM dTTP, and 3mM MgCl<sub>2</sub> | ||
<br><br><b>4.</b> < | <br><br><b>4.</b><br> | ||
{| class="wikitable" border="1" | {| class="wikitable" border="1" | ||
|- | |- |
Revision as of 11:06, 15 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAMLAB 1 WRITE-UP
Initial Machine TestingThe images above depict our OpenPCR machine. It performs polymerase chain reactions (PCR), a process in which a particular DNA sequence is amplified. The DNA sequence can then be easily analyzed. The OpenPCR machine accomplishes the amplification of a DNA sequence through a series of heating and cooling sequences.
When we unplugged the LCD screen from the circuit board, the machine stopped displaying information on the LCD screen. When we unplugged the white wire that connects the circuit board to the heating block, the heating block would not heat up.
We first tested our OpenPCR machine (machine #6) on October 25, 2012. We ran the machine through a preprogrammed sample test run. At first we were not getting consistent temperature readings outputting to the LCD screen; however, after we rebooted the OpenPCR machine and ran the test again the machine worked perfectly.
Protocols
Polymerase Chain Reaction
Research and DevelopmentSpecific Cancer Marker Detection - The Underlying Technology
(image source:http://www.nature.com/scitable/topicpage/the-biotechnology-revolution-pcr-and-the-use-553)
ResultsImageJ Software Processing
|