BME103:T130 Group 5 l2: Difference between revisions

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|10 ft Extension cord
|10 ft Extension cord
|1
|1
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{|border="1" cellpadding="5"
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! scope="col" | Supplied by the User
! scope="col" | Amount
|-
|Standard sized test tubes
|16
|-
|DNA Primer
|Amounts vary per experiment
|-
|DNA Samples
|Amounts vary per experiment
|-
|Computer
|1
|-
|Pipettes
|16
|Sybr Green
|Amounts vary per experiment
| Refrigerator
|1
|Power source
|N/A
|}
|}


'''PCR Protocol'''
'''PCR Protocol'''


 
<!--- Create a step-by-step procedure for setting up and running PCR reactions. Your instructions should include everything from adding reagents to the tubes, to programming the PCR machine and running the reaction.




Revision as of 15:25, 15 November 2012

BME 103 Fall 2012 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help

OUR TEAM

Name: Kyle Labban
Protocol Planner
Name: Haylee Poncy
Protocol Planner
Name: Liann Klein
Machine Engineer
Name: Wade Patrick
Machine Engineer
Name: Alexandria Lam
Research and Development Scientist

LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design


Key Features


Instructions





Protocols

Materials

Supplied in the Kit Amount
PCR Machine 1
Extra screws 5
CD containing programming application 1
Operations instruction manual 1
10 ft Extension cord 1


Supplied by the User Amount
Standard sized test tubes 16
DNA Primer Amounts vary per experiment
DNA Samples Amounts vary per experiment
Computer 1
Pipettes 16 Sybr Green Amounts vary per experiment Refrigerator 1 Power source N/A

PCR Protocol


Background on Disease Markers



Primer Design



Illustration


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