BME103:T130 Group 4 l2: Difference between revisions
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# Using the SYBR Green I transfer pipette, place one droplet of SYBR Green I in each of the middle wells of the first two rows on the glass slide. The two droplets should combine into one big drop. | # Using the SYBR Green I transfer pipette, place one droplet of SYBR Green I in each of the middle wells of the first two rows on the glass slide. The two droplets should combine into one big drop. | ||
# Using the corresponding transfer pipette, add two drops of the DNA sample you are testing to the SYBR Green I drop. | # Using the corresponding transfer pipette, add two drops of the DNA sample you are testing to the SYBR Green I drop. | ||
# Adjust your smartphone camera's settings as follows: turn off the flash, set the ISO to ≥800, set the white balance to auto, set exposure and saturation to their highest settings, and set contrast to the lowest setting. | # Adjust your smartphone camera's settings as follows: turn off the flash, set the ISO to ≥800, set the white balance to auto, set exposure and saturation to their highest settings, and set contrast to the lowest setting. Stand the smartphone upright in the cradle and set the cradle a few inches in front of the fluorimeter at a right angle to the slide. | ||
# Place the light box over the entire setup and turn on the fluorimeter's light. | # Place the light box over the entire setup and turn on the fluorimeter's light. | ||
# If the smartphone has a timer setting, set it and shut the light box so photos of the drop can be taken in complete darkness. Otherwise, keep the flap lowered as much as possible while you manually take photos. | # If the smartphone has a timer setting, set it and shut the light box so photos of the drop can be taken in complete darkness. Otherwise, keep the flap lowered as much as possible while you manually take photos. | ||
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# Navigate to <b>Analyze>Measure</b> and write down the sample numbers and measurement values. | # Navigate to <b>Analyze>Measure</b> and write down the sample numbers and measurement values. | ||
# To get a reading of the background noise, draw another oval of the same size above the drop in the Green image and navigate to <b>Analyze>Measure</b>. Write down the sample numbers and measurement values, and be sure to distinguish them from the original drop measurements. | # To get a reading of the background noise, draw another oval of the same size above the drop in the Green image and navigate to <b>Analyze>Measure</b>. Write down the sample numbers and measurement values, and be sure to distinguish them from the original drop measurements. | ||
# Repeat Steps 12-17 for each photo needing processing. | |||
==Research and Development== | ==Research and Development== |
Revision as of 22:01, 23 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features
Instructions
ProtocolsMaterials
Research and DevelopmentBackground on Disease Markers The marker that is being used is rs137852453. This SNP is associated with hemophilia. Hemophilia is a condition where someone's blood is not clotting properly. Data on this particular SNP variance can be seen here: http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=137852453
Bottom Primer (in reverse):
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