BME103:T130 Group 3 l2: Difference between revisions
Line 48: | Line 48: | ||
<font size=3><b>Materialsl</b></font><br><br> | <font size=3><b>Materialsl</b></font><br><br> | ||
{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|''' | | align="center" style="background:#f0f0f0;"|'''Supplied in the Kit''' | ||
|- | |- | ||
| | | PCR Machine | ||
|- | |- | ||
| 10μM forward primer | | 10μM forward primer | ||
|- | |- | ||
| 10μM reverse primer | | 10μM reverse primer | ||
|- | |- | ||
| GoTaq master mix | | GoTaq master mix | ||
|} | |} | ||
<font size=3><b>PCR Protocol</b></font><br><br> | |||
1.) The DNA samples were heated to ninety-five degrees Celsius (95°C) for one (1) minute to unzip the two single strands. <br> | |||
2.) They were then cooled to fifty-seven degrees Celsius (57°C) for ten (10) seconds and the primers were attached to their matching sequences. <br> | |||
3.) They were then heated back to seventy-two degrees Celsius (72°C) for ten (10) seconds and polymerase extended the DNA strands by attaching the correct free nucleotides in order on the single strands. <br><br> | |||
<font size=3>'''DNA Measurement Protocol'''</font><br><br> | <font size=3>'''DNA Measurement Protocol'''</font><br><br> |
Revision as of 15:30, 15 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features
Instructions
ProtocolsMaterialsl
PCR Protocol 1.) The DNA samples were heated to ninety-five degrees Celsius (95°C) for one (1) minute to unzip the two single strands. DNA Measurement Protocol Fluorimeter Setup Fluorimeter Measurements ImageJ Instructions Research and DevelopmentBackground on Disease Markers
Primer Design
Illustration
|